Anti-HMGCS1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q01581

Core Product Specifications and Parameters

Biological Background: HMGCS1 Function and Localization

• HMGCS1, also known as Hydroxymethylglutaryl-CoA synthase cytoplasmic or 3-hydroxy-3-methylglutaryl coenzyme A synthase, is a key enzyme in cholesterol biosynthesis.
• It catalyzes the condensation of acetyl-CoA and acetoacetyl-CoA to form HMG-CoA, which is then converted to mevalonate by HMG-CoA reductase (HMGCR) as a precursor for cholesterol synthesis.
• The enzyme localizes predominantly in the cytoplasm.
• It is highly expressed in the cerebral cortex, suggesting a role in brain cholesterol metabolism.
• Classified as a transferase involved in lipid and sterol metabolism; subject to post-translational modifications including phosphorylation and acetylation.
• Associated with disease variants according to UniProt keywords, indicating potential clinical relevance in metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fusion protein covering amino acids 241–520 of human HMGCS1 (NP_002121.4). Consider this region for any blocking or epitope mapping studies.
• This polyclonal antibody is suitable for WB, IF/ICC, and ELISA applications; optimize dilutions and conditions for your specific assay.
• Reported cross-reactivity with human, mouse, and rat allows flexibility in model organism selection. Validate in the relevant sample system, especially tissues with high HMGCS1 expression such as brain.
• Use appropriate controls (e.g., HMGCS1-overexpressing lysates or knockdown cells) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, supporting diagnostic manufacturers and researchers from concept to clinic. For your cholesterol and lipid metabolism projects, we offer validated rabbit polyclonal antibodies like this anti-HMGCS1 pAb, along with optimized monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Whether you need custom antibody pairs or ready-to-use immunoassay components, CamelBio streamlines procurement with reliable supply and technical expertise.