Anti-HMGCR Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04035

Core Product Specifications and Parameters

Biological Background: HMGCR Function and Localization

• HMGCR (3-hydroxy-3-methylglutaryl-coenzyme A reductase) catalyzes the conversion of HMG-CoA to mevalonate, the rate-limiting step in cholesterol and isoprenoid biosynthesis, thus playing a critical role in cellular cholesterol homeostasis. Related references: PMID:21357570, PMID:2991281, PMID:36745799

• It is the primary molecular target of statins, a class of cholesterol-lowering drugs. Related references: PMID:11349148, PMID:18540668, PMID:36745799

• HMGCR localizes predominantly to the endoplasmic reticulum membrane and the peroxisome membrane, consistent with its role in lipid metabolism.

• It is ubiquitously expressed, with the highest levels detected in the cerebellum, fetal brain, testis, skin, and adrenal gland.

• The protein undergoes post-translational modifications including glycosylation and phosphorylation, and is annotated with keywords indicating its involvement in cholesterol metabolism, steroid biosynthesis, and alternative splicing.

• Mutations in HMGCR are associated with limb-girdle muscular dystrophy, highlighting its importance in muscle function.

Experimental Guidance and Technical Tips

• The antibody was raised against a C-terminal peptide (aa 731-830); expect detection of the full-length ~97 kDa protein in WB.
• For WB, consider using reducing conditions and loading appropriate positive control lysates from tissues with high HMGCR expression (e.g., liver, brain).
• For IHC-P, perform antigen retrieval and validate staining in known positive tissues; include negative controls to ensure specificity.
• Due to the polyclonal nature, we recommend re-validation when switching to a new production lot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials, including validated anti-HMGCR polyclonal antibodies for cholesterol metabolism and statin research. Our sourcing network covers a wide range of ancillary reagents and bulk antibody pairs, supporting your development needs from early-stage research to clinical assay validation. For rare targets or customized formulations, our technical team is ready to assist with tailored procurement solutions.