Anti-HMGCL Polyclonal Antibody for WB, IHC-P, ELISA - P35914

Core Product Specifications and Parameters

Biological Background: HMGCL Function and Localization

• HMGCL (Hydroxymethylglutaryl-CoA lyase, mitochondrial) is a key enzyme in ketogenesis and leucine catabolism, catalyzing the cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate.
• It localizes primarily to the mitochondrial matrix and is also found in peroxisomes, consistent with its role in lipid metabolism.
• The enzyme has the highest expression in liver, with additional expression in pancreas, kidney, intestine, testis, fibroblasts, and lymphoblasts; very low expression in brain and skeletal muscle.
• An alternative splicing isoform (isoform 2) shows highest relative mRNA expression in heart (30%), skeletal muscle (22%), and brain (14%).
• HMGCL is a metal-binding lyase; its activity depends on divalent cations, and it belongs to the HMG-CoA lyase family.
• Post-translational modifications include acetylation and disulfide bond formation, which may regulate its function or stability.
• Mutations in HMGCL are linked to 3-hydroxy-3-methylglutaryl-CoA lyase deficiency, a rare autosomal recessive metabolic disorder.
• Ketone bodies produced via this pathway serve as an alternative energy source to glucose, especially during fasting, and act as lipid precursors and metabolic regulators.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human HMGCL (aa 1-325), so the antibody is expected to recognize the native protein in denaturing and native conditions.
• For Western blot, a band at approximately 34 kDa is anticipated; consider using reducing conditions and validate with a positive control such as liver lysate.
• In IHC-P, anticipate mitochondrial staining in tissues with high HMGCL expression (e.g., liver); an appropriate antigen retrieval method may be necessary.
• The antibody cross-reacts with mouse and rat; when using these species, validate specificity in the relevant tissue or cell system.
• For ELISA, consider coating with the recombinant immunogen or a peptide and optimize the assay conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, research laboratories, and institutes with a comprehensive one-stop platform for IVD raw materials. This anti-HMGCL polyclonal antibody supports research into ketogenesis, leucine metabolism, and related metabolic disorders, and is backed by our capability to supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your entire development workflow from concept to clinic.