Anti-HMGB3 Rabbit Polyclonal Antibody for WB and ELISA - O15347

Core Product Specifications and Parameters

Biological Background: HMGB3 Function and Localization

• HMGB3 (high mobility group protein B3), also known as HMG-4, HMG-2a, or HMG2A, is a member of the HMGB family of chromosomal proteins.
• This multifunctional protein associates with chromatin and binds DNA, preferring non-canonical structures such as single-stranded DNA. It can bend DNA and enhance DNA flexibility, facilitating activities on gene promoters.
• In the cytoplasm, HMGB3 may act as a promiscuous immunogenic sensor for DNA and RNA, contributing to the innate immune response to nucleic acids.
• It negatively regulates B-cell and myeloid cell differentiation, and in hematopoietic stem cells, it helps balance self-renewal versus differentiation.
• HMGB3 is involved in the negative regulation of canonical Wnt signaling.
• Subcellular localization includes the nucleus, chromosomes, and cytoplasm.
• Post-translational modifications noted for HMGB3 include acetylation, phosphorylation, oxidation, and disulfide bond formation.
• Expressed predominantly in the placenta.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1–100 of human HMGB3. Given the polyclonal nature, the antibody may recognize multiple isoforms or closely related proteins; validate specificity in your sample system.
• For Western blot, the predicted molecular weight is 23 kDa, though post-translational modifications may shift the apparent band size. Consider using reducing conditions and loading appropriate controls.
• In ELISA, optimize antibody dilution and employ a suitable blocking buffer to minimize background.
• Cross-reactivity with rat has been confirmed; for other species, check sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This HMGB3 rabbit polyclonal antibody, suitable for WB and ELISA, exemplifies our comprehensive offerings for chromatin and innate immunity research. Let CamelBio be your sourcing bridge to streamline assay development and procurement, covering every stage from concept to clinic.