Anti-HMGB2 Rabbit Polyclonal Antibody for WB, ELISA - P26583

Core Product Specifications and Parameters

Biological Background: HMGB2 Function and Localization

• HMGB2 binds DNA with a preference for non-canonical structures, bending DNA and enhancing DNA flexibility to promote transcription factor binding and long-range regulatory interactions.
  Related references:
  PMID:11909973
  PMID:18413230
  PMID:19522541

• It serves as a cofactor for the RAG complex, stimulating V(D)J recombination by enhancing cleavage and RAG protein binding at recombination signal sequences.

• Extracellular HMGB2 acts as a chemokine, promoting endothelial cell proliferation and migration via AGER/RAGE.
  Related references:
  PMID:19811285

• Exhibits antimicrobial activity in gastrointestinal epithelial tissues.
  Related references:
  PMID:23877675

• Plays roles in innate immune sensing of nucleic acids, inflammatory response, and neurogenesis modulation.

• Predominantly nuclear and chromosomal, but also detected in cytoplasm and secreted.

• Expressed in gastric and intestinal tissues at the protein level.

• Subject to acetylation, phosphorylation, and oxidation; contains disulfide bonds, suggesting redox-sensitive regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–300 of human HMGB2; consider validating antibody specificity by peptide competition or knockout controls in your assay system.
• For WB, the predicted molecular weight is 24 kDa; use appropriate gel percentage and verify the band in relevant cell/tissue lysates known to express HMGB2 (e.g., gastrointestinal tissues).
• Cross-reactivity with mouse and rat allows comparative studies across species; confirm performance under your specific experimental conditions.
• This antibody is suitable for ELISA; optimize coating and detection conditions for your target concentration range.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering one-stop access to validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents for manufacturing. The HMGB2 antibody supports research in chromatin biology and inflammation, bridging discovery and diagnostic development. Through CamelBio’s supply network, diagnostic manufacturers can source rare-target raw materials and scale production with confidence.