Anti-HMGB2 Polyclonal Antibody for WB, IF/ICC, ELISA - P26583

Core Product Specifications and Parameters

Biological Background: HMGB2 Function and Localization

• HMGB2 (High mobility group protein B2, also known as HMG-2) binds DNA with preference for non-canonical structures, bends DNA, and enhances DNA flexibility to promote transcription factor binding and bring distant regulatory sequences into proximity. Related references: PMID:11909973, PMID:18413230, PMID:19522541
• Acts as a cofactor of the RAG complex in V(D)J recombination, stimulating cleavage and RAG protein binding at recombination signal sequences.
• Proposed to function as a promiscuous immunogenic DNA/RNA sensor in the innate immune response to nucleic acids, cooperating with specific pattern recognition receptors.
• In the extracellular compartment, acts as a chemokine that promotes proliferation and migration of endothelial cells via AGER/RAGE. Related references: PMID:19811285
• Exhibits antimicrobial activity in gastrointestinal epithelial tissues. Related references: PMID:23877675
• Subcellular localization includes nucleus, chromosome, cytoplasm, and secretion.
• Expressed in gastric and intestinal tissues at the protein level.
• Post-translational modifications include acetylation, phosphorylation, oxidation, and disulfide bond formation, relating to chemotaxis, DNA recombination, transcription regulation, and inflammatory response.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-200 of human HMGB2; consider verifying sequence conservation in your target species.
• This polyclonal antibody may be suitable for WB, IF/ICC, and ELISA; optimize dilutions experimentally.
• Because HMGB2 localizes to both nucleus and cytoplasm, IF/ICC protocols may require appropriate fixation and permeabilization.
• Cross-reactivity is expected with mouse and rat HMGB2; validate in relevant sample systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For your HMGB2-related studies on chromatin dynamics, transcription, and innate immunity, our platform streamlines sourcing from concept to clinic. We also specialize in rare-target raw-material procurement and technical consulting.