Anti-HMGB1 Rabbit Monoclonal Antibody (KO Validated) for WB, IHC-P, IF/ICC, ELISA - P09429

Core Product Specifications and Parameters

Biological Background: High mobility group protein B1 Function and Localization

• HMGB1 (high mobility group protein B1, also known as HMG-1) is a multifunctional redox-sensitive protein that acts as a DNA chaperone in the nucleus, where it participates in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair, and genome stability. (PubMed:33147444) Related references: PMID:33147444
• It functions as a danger-associated molecular pattern (DAMP) when released extracellularly, mediating inflammatory and immune responses by binding to receptors such as RAGE, TLR2, and TLR4. (PubMed:27362237, PubMed:34743181) Related references: PMID:27362237 PMID:34743181
• The activity of HMGB1 is dictated by its redox state: the fully reduced form acts as a chemokine, the disulfide form as a cytokine, and the sulfonyl form promotes immunological tolerance. (PubMed:23446148, PubMed:23519706, PubMed:23994764) Related references: PMID:23446148 PMID:23519706 PMID:23994764
• Subcellular localization of HMGB1 is dynamic and includes the nucleus, chromosome, cytoplasm, cell membrane, endosome, endoplasmic reticulum, and secreted forms.
• Post-translational modifications such as acetylation, ADP-ribosylation, phosphorylation, and oxidation regulate HMGB1's subcellular distribution and functional activities.
• HMGB1 is ubiquitously expressed, with confirmed expression in platelets. (PubMed:11154118) Related references: PMID:11154118
• The protein plays a role in host-virus interactions, being critical for entry of SARS-CoV-2 and SARS-CoV, and facilitating replication of influenza A, EBV, and dengue virus. (PubMed:33147444, PubMed:35239449) Related references: PMID:33147444 PMID:35239449

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100–200 of human HMGB1. Consider using full-length recombinant protein or epitope mapping to confirm detection specificity.
• For Western blotting, HMGB1 has a predicted molecular weight of ~25 kDa; multiple bands may appear due to post-translational modifications or redox states.
• In IHC-P, optimize antigen retrieval methods and validate using tissues with known HMGB1 expression patterns (e.g., tonsil, liver). Include appropriate positive and negative controls.
• In IF/ICC, note that HMGB1 can shuttle between nucleus and cytoplasm depending on cellular stress. Treat cells with stimuli such as LPS or TNF-α to assess translocation.
• For ELISA, ensure the antibody pair is validated for the chosen assay format; the epitope region (100–200) should be accessible in the target antigen preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing tailored for projects involving chromatin biology, inflammatory markers, and autophagy research. Whether you need high-quality anti-HMGB1 antibodies or complementary reagents, CamelBio is your reliable B2B partner for accelerating assay development and commercialization.