Anti-HMGA2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P52926

Core Product Specifications and Parameters

Biological Background: HMGA2 Function and Localization

• HMGA2, also known as High mobility group protein HMGI-C or High mobility group AT-hook protein 2, is a member of the HMGA family of DNA-binding proteins.
• It functions as a transcriptional regulator, playing a key role in cell cycle progression through the regulation of CCNA2 expression.
• HMGA2 is involved in chromosome condensation during the meiotic G2/M transition of spermatocytes, highlighting its role in cell division and DNA condensation.
• In postnatal myogenesis, HMGA2 promotes satellite cell activation, contributing to muscle regeneration and growth (By similarity).
• It positively regulates IGF2 expression both dependently and independently of PLAG1 transcription factor. Related references: PMID:28796236
• Subcellularly, HMGA2 localizes to the nucleus, consistent with its role in DNA binding and transcriptional modulation.
• Post-translational modifications include acetylation and phosphorylation, and the protein undergoes alternative splicing, indicating regulatory complexity.
• Clinically, HMGA2 is associated with chromosomal rearrangements and functions as a proto-oncogene, with disease variants linked to dwarfism and growth abnormalities.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 55–109 of human HMGA2. For Western blot, anticipate a band near 12 kDa; validate specificity using knockout or knockdown controls.
• For immunohistochemistry (IHC-P), expect nuclear staining. Include DAPI co-staining to confirm subcellular localization and use antigen retrieval protocols optimized for nuclear antigens.
• In ELISA applications, this antibody can be employed as a capture or detection reagent. Optimal dilutions and buffer conditions should be determined empirically.
• Cross-reactivity to mouse and rat is indicated, but performance should be verified in the relevant model systems.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, bulk ancillary reagents, and custom procurement for specialized targets such as HMGA2. Our platform connects diagnostic manufacturers and research laboratories with reliable supplies to streamline assay development from concept to clinic.