Anti-HMGA2 Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - P52926

Core Product Specifications and Parameters

Biological Background: HMGA2 Function and Localization

• HMGA2, also known as high mobility group protein HMGI-C, belongs to the HMGA family of architectural transcription factors characterized by multiple AT-hook DNA-binding domains.
• It functions as a transcriptional regulator, modulating gene expression by binding to AT-rich regions in the minor groove of DNA, thereby affecting chromatin structure and gene accessibility.
• HMGA2 plays a critical role in cell cycle regulation, particularly through CCNA2, and is essential for chromosome condensation during the meiotic G2/M transition in spermatocytes.
• The protein is involved in postnatal myogenesis and is required for satellite cell activation, contributing to muscle regeneration and growth.
• HMGA2 positively regulates the expression of insulin-like growth factor 2 (IGF2) via both PLAG1-dependent and PLAG1-independent mechanisms.
  Related references:
  PMID:28796236
• Subcellularly, HMGA2 is predominantly localized to the nucleus, consistent with its roles in transcriptional regulation, DNA condensation, and chromatin organization.
• Post-translational modifications, including acetylation and phosphorylation, and its involvement in chromosomal rearrangements and proto-oncogenic activities, highlight HMGA2's multifaceted regulation and significance in growth and disease.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human HMGA2, which encompasses key AT-hook domains critical for DNA interaction.
• Suitable applications include Western blot (WB), immunohistochemistry (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA; always validate the antibody in your specific experimental system.
• Given the predicted molecular weight of ~12 kDa, ensure optimal gel percentage and transfer conditions for low-molecular-weight proteins in WB.
• For IF/ICC, as HMGA2 is a nuclear protein, consider appropriate fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) protocols to achieve clear nuclear staining.
• The antibody shows cross-reactivity with human and mouse samples; if using other species, preliminary testing is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers, laboratories, and research institutes with streamlined access to high-quality reagents like this HMGA2 Rabbit mAb. Our supply network encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing, supporting your cell cycle, transcriptional regulation, and oncology research from concept to clinic.