Anti-HMGA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P17096

Core Product Specifications and Parameters

Biological Background: HMGA1 Function and Localization

• HMGA1 encodes two major isoforms, HMG-I and HMG-Y, through alternative splicing; both function as architectural transcription factors that remodel chromatin.
• The protein binds preferentially to the minor groove of AT-rich regions in double-stranded DNA, modulating DNA flexibility and influencing the assembly of transcriptional complexes.
• It plays a role in nucleosome phasing and higher-order chromatin organization, thereby regulating the expression of genes near AT-rich sequences.
• Subcellularly, HMGA1 localizes to the nucleus and associates with chromosomes, consistent with its DNA-binding and gene-regulatory functions.
• Post-translational modifications—including acetylation, phosphorylation, ADP-ribosylation, and methylation—dynamically regulate its DNA-binding affinity and transcriptional activity.
• The protein is a target of chromosomal rearrangements in various cancers, and its overexpression is linked to tumor progression and metastasis.
• Ubiquitin-like conjugation (SUMOylation) and isopeptide bond formation also modulate HMGA1 stability and localization, adding layers of regulation to its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human HMGA1; for applications like WB, consider using nuclear-enriched lysates to enhance detection of this low-abundance nuclear protein.
• For IHC-P, optimize antigen retrieval conditions and validate the antibody with appropriate positive and negative tissue controls; nuclear staining is expected.
• When performing ELISA, use the immunizing peptide as a positive control to confirm binding specificity.
• Cross-reactivity with mouse HMGA1 has been reported; if using mouse samples, verify specificity in the target experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with comprehensive IVD raw material solutions—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents. For those working on transcription regulation, chromatin biology, or cancer biomarker development, we provide rapid access to rare-target raw materials like HMGA1 antibodies to accelerate your project from concept to clinic.