Anti-HMGA1 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA, ChIP-seq - P17096

Core Product Specifications and Parameters

Biological Background: HMGA1 Function and Localization

• HMGA1, also known as high mobility group protein HMG-I/HMG-Y or AT-hook protein 1, is a non-histone chromosomal protein encoded by the HMGA1 gene (GeneID: 3159). Alternative splicing produces multiple isoforms.
• It binds preferentially to the minor groove of A+T-rich double-stranded DNA, functioning in nucleosome phasing and chromatin organization.
• HMGA1 is involved in the transcriptional regulation of genes containing or positioned near A+T-rich regions, influencing gene expression.
• The protein localizes to the nucleus and chromosomes, as indicated by its subcellular location.
• Post-translational modifications include acetylation, ADP-ribosylation, methylation, phosphorylation, and ubiquitin-like conjugation, which may modulate its DNA-binding and regulatory activities.
• HMGA1 has been implicated in chromosomal rearrangements, highlighting its dynamic role in genome stability.
• The molecular weight of the protein is approximately 11.7 kDa (calculated from sequence), though it may migrate at around 12 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 1–107 of human HMGA1 (P17096). For Western blot, consider that the predicted molecular weight is ~12 kDa; use appropriate gel percentage and transfer conditions for low-molecular-weight proteins.
• For ChIP-seq, validate antibody specificity using knockout or knockdown controls, as HMGA1 binds broadly to A+T-rich DNA.
• When performing immunofluorescence, ensure permeabilization to access nuclear targets; note that HMGA1 localizes to the nucleus and chromosomes.
• For IHC-P, antigen retrieval may be required; optimize using positive control tissues known to express HMGA1, such as proliferative tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with one-stop access to validated IVD raw materials. This HMGA1 antibody is part of our broad catalog for chromatin biology and transcription research. We supply optimized monoclonal and polyclonal antibodies, bulk reagents, and rare-target sourcing to accelerate your assay development from concept to clinic.