Anti-HMG20B Rabbit Polyclonal Antibody [KO Validated] for WB and ELISA - Q9P0W2

Core Product Specifications and Parameters

Biological Background: HMG20B Function and Localization

• HMG20B (also known as BRAF35, HMGX2, SMARCE1r) is a HMG box-containing protein belonging to the SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1-related family. It undergoes alternative splicing, producing multiple isoforms.
• The protein plays a critical role in cell cycle progression, specifically required for the G2 phase and entry into mitosis. It is also essential for RCOR1/CoREST-mediated repression of neuronal-specific gene promoters.
• HMG20B contains an HMG-box domain that mediates DNA binding and facilitates chromatin interactions, contributing to transcriptional regulation and chromatin remodeling.
• Subcellular localization is predominantly nuclear, associating with chromosomes, consistent with its function in chromatin organization and gene silencing.
• The protein is subject to post-translational modifications, including phosphorylation and isopeptide bond formation (ubiquitination), which may regulate its stability and activity.
• Ubiquitously expressed in adult tissues, HMG20B is implicated in fundamental cellular processes, as reflected by its annotation with keywords such as "Cell cycle", "Chromatin regulator", and "Transcription regulation".

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal 317 amino acids of human HMG20B, which includes the HMG-box domain. This region is critical for DNA binding and chromatin association, suggesting that the antibody recognizes the functional domain.
• For Western blot, consider using whole-cell or nuclear extracts, and expect a band around 36 kDa. Validate in relevant human, mouse, or rat cell lines according to the reported cross-reactivity.
• In ELISA applications, consider pairing this polyclonal antibody with a monoclonal detection antibody targeting a distinct epitope to enhance specificity. Optimization of assay conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering reliable access to validated IVD raw materials such as this anti-HMG20B polyclonal antibody. Beyond this product, we provide support with antibody pairs, bulk ancillary reagents, and sourcing for rare target-specific tools, streamlining supply chains for chromatin biology, cell cycle, and transcription regulation applications. Our team collaborates with you from initial development to clinical implementation, ensuring a seamless procurement experience.