Anti-HMG20B Rabbit Polyclonal Antibody for WB, ELISA - Q9P0W2

Core Product Specifications and Parameters

Biological Background: HMG20B Function and Localization

• HMG20B (BRAF35, SOXL) is a high mobility group (HMG) box-containing protein that acts as a chromatin regulator, also known as SMARCE1r.
• It plays a critical role in cell cycle progression, specifically required for correct G2 phase transit and entry into mitosis.
• Functions as a corepressor for RCOR1/CoREST-mediated transcriptional repression of neuronal-specific gene promoters.
• Localizes to the nucleus and is associated with chromosomes, consistent with its role in chromatin organization and transcription regulation.
• Ubiquitously expressed across adult tissues, indicating a general regulatory function.
• Subject to post-translational modifications, including phosphorylation and ubiquitination (isopeptide bond formation), suggesting regulation of its activity.
• The protein undergoes alternative splicing, generating multiple isoforms with potentially distinct functions.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-317) encompasses the N-terminal region, which includes the HMG box domain; consider verifying band specificity in target cell lines using siRNA knockdown or known HMG20B-negative models.
• For Western blot, a band around 36 kDa is expected based on the calculated molecular weight; however, validation in relevant human and mouse tissue or cell lysates is recommended.
• In ELISA, the recombinant immunogen protein can be used as a positive control to assess antibody binding and set up standard curves.
• As a rabbit polyclonal antibody, performance may vary between batches; it is advisable to include appropriate controls in each experiment.
• Given the ubiquitous expression, background signals may be observed in whole-cell lysates; optimizing blocking and washing conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, bridging the gap between research and clinical diagnostics. This anti-HMG20B polyclonal antibody exemplifies our commitment to supplying validated reagents for chromatin biology, cell cycle, and transcription research. Explore our extensive portfolio for bulk antibodies, matched pairs, and customized rare-target sourcing to accelerate your assay development.