Anti-HMG20A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NP66

Core Product Specifications and Parameters

Biological Background: HMG20A Function and Localization

• HMG20A, also known as HMGX1 or HMGXB1, is a high mobility group box-containing protein that belongs to the HMG20 family of chromatin regulators.
• It functions as a DNA-binding protein and chromatin regulator, playing a critical role in neuronal differentiation by modulating gene expression.
• HMG20A acts as an inhibitor of HMG20B and overcomes the repressive effects of the neuronal silencer REST, thereby promoting the activation of neuronal-specific genes.
• It is involved in recruiting the histone methyltransferase KMT2A/MLL1, leading to increased trimethylation of histone H3 at lysine 4 (H3K4me3), a mark of active transcription.
• The protein localizes exclusively to the nucleus, consistent with its role in transcriptional regulation and chromatin remodeling.
• HMG20A is ubiquitously expressed in human tissues, indicating broad regulatory functions beyond neurogenesis.
• It contains a coiled-coil domain and is subject to phosphorylation, suggesting regulation by signaling pathways; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of HMG20A is approximately 40 kDa. Be aware that post-translational modifications or isoform variations may cause band shifts; validate with appropriate controls.
• In immunofluorescence or ICC, expect nuclear staining. Use a suitable fixation and permeabilization protocol tailored to your cell type to ensure epitope accessibility.
• This polyclonal antibody is raised against a recombinant protein covering the N-terminal region (aa 1-347). For ELISA applications, consider pairing with a detection antibody that recognizes a non-overlapping epitope for sandwich assays.
• As the antibody cross-reacts with mouse and rat, it can be used for comparative studies across species, but always verify specificity in your experimental system by including knockout or knockdown controls when possible.
• Since this is a polyclonal antibody, lot-to-lot consistency may vary; for critical quantitative assays, we recommend titrating each new lot and running replicate experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline IVD raw material procurement, from early R&D to clinical applications. Our catalog includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support chromatin biology and neuroscience research. Whether you need rare target reagents like HMG20A antibodies or scalable sourcing solutions, CamelBio is committed to bridging your concept to clinic with reliable supply and technical support.