Anti-HMBS Polyclonal Antibody for WB, ELISA - P08397

Core Product Specifications and Parameters

Biological Background: HMBS Function and Localization

• HMBS, also known as porphobilinogen deaminase (PBGD) or hydroxymethylbilane synthase, is a key enzyme in the heme biosynthetic pathway.
• It catalyzes the polymerization of four molecules of porphobilinogen to form hydroxymethylbilane (preuroporphyrinogen). Related references: PMID:18004775, PMID:18936296, PMID:19138865
• The enzyme assembles a dipyrromethane cofactor from two porphobilinogen molecules, which serves as a primer for tetrapyrrole assembly. Related references: PMID:18936296
• HMBS is localized to the cytoplasm, specifically the cytosol.
• It is ubiquitously expressed, with a specific presence in erythroid cells.
• Post-translational modifications include acetylation and phosphorylation, and the protein is subject to alternative splicing.
• Mutations in HMBS are associated with acute intermittent porphyria.

Experimental Guidance and Technical Tips

• For Western blot, consider starting with a 1:500-1:2000 dilution and validating with positive control lysates from human or mouse cells known to express HMBS.
• In ELISA, the antibody can be used as capture or detection reagent; optimization of coating and detection concentrations is recommended.
• Given the full-length immunogen, the antibody may detect multiple HMBS isoforms; confirm specificity by comparing with known molecular weights (predicted ~39 kDa).
• Store at -20°C upon receipt and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies. For HMBS and heme biosynthesis research, we offer ready-to-use antibody pairs, optimized reagents for WB/ELISA, and bulk ancillary materials. Our expert team can also assist in sourcing rare targets and custom antibody development to support your project from concept to clinic.