Anti-HLX Rabbit Polyclonal Antibody for WB, ELISA - Q14774

Core Product Specifications and Parameters

Biological Background: HLX Function and Localization

• HLX (H2.0-like homeobox protein), also known as HB24 or HLX1, is a homeobox transcription factor encoded by the HLX gene.
• It functions as a critical transcription factor required for TBX21/T-bet-dependent maturation of Th1 cells and maintenance of Th1-specific gene expression.
• HLX is involved in embryogenesis and hematopoiesis, playing a role in developmental processes.
• The protein localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• Tissue expression: low in normal B and T-cells, but high in activated lymphocytes and monocytes; also present in thymus, tonsil, bone marrow, developing vessels, and fetal brain.
• It contains a homeobox DNA-binding domain, classifying it within the homeobox protein family, which regulates differentiation and development.
• Keywords from UniProt include Differentiation, DNA-binding, Homeobox, Nucleus, Proteomics identification, Reference proteome, Transcription, and Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 80–280 of human HLX, which likely contains the homeobox domain. Consider performing WB on nuclear extracts to capture the transcription factor in its native location.
• Given HLX expression is elevated in activated lymphocytes, positive control samples from stimulated immune cells may be useful for validation.
• For ELISA, recombinant HLX protein can be used as coating antigen to assess antibody binding; however, validate in the relevant sample system.
• Assess cross-reactivity with other homeobox family members if off-target binding is a concern, as the immunogen region may share homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibodies like this Anti-HLX polyclonal antibody, as well as monoclonal antibodies, antigen pairs, and ancillary reagents for immunoassay development in immunology and transcription research. For rare targets and custom sourcing needs, CamelBio offers tailored solutions to accelerate your research and development.