Anti-HLTF Rabbit Polyclonal Antibody for WB, ELISA - Q14527

Core Product Specifications and Parameters

Biological Background: HLTF Function and Localization

• HLTF (Helicase-like transcription factor, also known as SMARCA3, RNF80, HIP116, SNF2L3) is a dual-function enzyme with DNA-dependent ATPase activity and E3 ubiquitin-protein ligase activity, involved in chromatin regulation and DNA damage tolerance (DDT).
  Related references: PMID:18316726, PMID:18719106, PMID:26051180

• It catalyzes 'Lys-63'-linked polyubiquitination of monoubiquitinated PCNA at 'Lys-164', promoting error-free postreplication repair via template switching.
  Related references: PMID:18316726, PMID:18719106

• HLTF binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and mediates histone H3 'Lys-23' polyubiquitination (H3K23ub), creating a docking site for DNMT1 to ensure replication-coupled DNA methylation maintenance.
  Related references: PMID:40680746

• As a chromatin remodeling factor, HLTF regulates transcription and exhibits ATP-dependent double-stranded DNA translocase activity without classical helicase function; it can mediate replication fork reversal to suppress DNA synthesis and maintain genomic stability.
  Related references: PMID:10391891, PMID:1994885, PMID:9126292

• HLTF cooperates with MSH2 to resolve G-quadruplex (G4) DNA structures, limiting replication stress and G4 accumulation throughout the cell cycle.
  Related references: PMID:39142279

• It contributes to nucleotide excision repair by evicting lesion-containing oligonucleotides via its HIRAN and ATPase domains, and can displace single-stranded DNA from triplex structures.
  Related references: PMID:26051180, PMID:31960921

• HLTF also has protein clearing activity at stalled replication forks, facilitating restart of DNA replication.
  Related references: PMID:21795603

• HLTF localizes to the nucleus and chromosomes, and is broadly expressed in tissues including brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 726–1009 of human HLTF, covering a C‑terminal region that includes the RING finger domain; this design may help generate antibodies recognizing full‑length HLTF and its key functional epitopes.
• For Western blot, the expected molecular weight of HLTF is approximately 114 kDa; validate the antibody in relevant human cell lines, as cross‑reactivity with other species has not been tested.
• This antibody is intended for ELISA and WB applications; consider optimizing antibody dilutions and assay conditions for your specific sample matrix and experimental system.
• Shipment is on ice bags; upon receipt, store the antibody at the recommended temperature (–20°C or –80°C) to maintain stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one‑stop access to IVD raw materials, technical services, and consulting. The HLTF rabbit polyclonal antibody supports research into DNA repair, chromatin remodeling, and epigenetic regulation—areas critical for cancer and genomic instability studies. Beyond single antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your development from concept to clinic.