Anti-HLA-DRB4 Rabbit Monoclonal Antibody for WB and ELISA - P13762

Core Product Specifications and Parameters

Biological Background: HLA-DRB4 Function and Localization

• Serves as the beta chain of MHC class II DR molecules, playing a central role in the adaptive immune system by binding peptides from endocytosed or autophagic sources and presenting them to CD4+ helper T cells.
• The peptide-binding cleft accommodates peptides of 10–30 residues derived mainly from proteins degraded in the endosomal/lysosomal pathway of antigen‑presenting cells (APCs) such as dendritic cells, macrophages, and B cells.
• Assembly begins in the endoplasmic reticulum by forming a heterononamer with CD74 (invariant chain), which directs the complex to antigen‑processing compartments and prevents premature peptide binding.
• In late endosomes/lysosomes, CD74 is sequentially cleaved by cathepsins, and the residual CLIP fragment is exchanged for high‑affinity antigenic peptides through the action of HLA‑DM; in B cells and dendritic cells, HLA‑DO modulates this exchange.
• Constitutively expressed on professional APCs and also found on gastrointestinal epithelial cells, where it contributes to local immune surveillance and oral tolerance.
• Cycles through multiple membrane compartments including the cell membrane, endoplasmic reticulum, Golgi network, endosomes, lysosomes, and late endosomes.
• Post‑translational modifications include N‑linked glycosylation, disulfide bonds, isopeptide bonds, and potential ubiquitination—all of which influence protein stability, conformation, and trafficking.
• Highly polymorphic and implicated in transplantation immunology; mismatches in donor and recipient HLA‑DR can trigger alloreactive T‑cell responses.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to amino acids 50–150 within the extracellular domain; for applications requiring native conformation (e.g., flow cytometry), verify that the epitope is accessible on live cells.
• Recommended applications include Western blot (WB) and ELISA; typical WB detection yields a band near 30 kDa. Optimize antibody dilutions using appropriate human cell lysates, such as Raji or Daudi B‑cell lines.
• Cross‑reactivity is demonstrated for human; ortholog reactivity in other species has not been characterized and should be tested individually.
• Genetic homology among DRB genes may lead to cross‑recognition; employ siRNA knockdown or CRISPR‑based knockout controls if isoform‑specific discrimination is critical for your study.
• Follow standard shipping and storage conditions: keep on ice bags, aliquot upon receipt, and avoid repeated freeze‑thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw‑material partner, CamelBio connects diagnostic manufacturers, laboratories, and research institutes with the reagents they need—from discovery through clinical deployment. The HLA‑DRB4 rabbit monoclonal antibody joins a growing catalog of validated antibodies and ancillary products for immunology and transplantation research. Whether you require established immunoassay components or custom sourcing of rare targets, CamelBio provides the reliable supply chain and technical support to accelerate your IVD development.