Anti-HLA-DRB3 Polyclonal Antibody for WB and ELISA - P79483

Core Product Specifications and Parameters

Biological Background: HLA-DRB3 Function and Localization

• HLA-DRB3 encodes the beta chain of the MHC class II molecule, which pairs with the alpha chain HLA-DRA to form a heterodimer on the surface of professional antigen-presenting cells (APCs).
• The MHCII complex presents peptide antigens (typically 10–30 amino acids from endocytosed proteins) to CD4+ T cells, guiding T-helper effector functions such as antibody-mediated immunity and macrophage activation (PubMed:16148104, PubMed:19531622, PubMed:19830726). Related references: PMID:16148104, PMID:19531622, PMID:19830726
• The gene has several alleles with distinct peptide-binding repertoires. ALLELE DRB3*01:01 presents tetanus toxin and HHV-6B epitopes (PubMed:19830726, PubMed:2463305, PubMed:2788702, PubMed:31020640). Related references: PMID:19830726, PMID:2463305, PMID:2788702, PMID:31020640
• ALLELE DRB3*02:02 presents epitopes from tetanus toxin, EBV, HHV-6B, dengue virus, IAV, and tumor antigens WT1, NY-ESO-1, and TP53 (PubMed:19830726, PubMed:2788702, PubMed:23569328, PubMed:31308093, PubMed:31020640, PubMed:31333679, PubMed:22929521, PubMed:19531622, PubMed:20368442, PubMed:30282837). Related references: PMID:19830726, PMID:2788702, PMID:23569328, PMID:31308093, PMID:31020640, PMID:31333679, PMID:22929521, PMID:19531622, PMID:20368442, PMID:30282837
• ALLELE DRB3*03:01 presents peptides from M. tuberculosis and HCV (PubMed:32341563, PubMed:2788702, PubMed:16148104). Related references: PMID:32341563, PMID:2788702, PMID:16148104
• Subcellularly, it localizes to the cell membrane as well as membranes of the endoplasmic reticulum, late endosomes, lysosomes, and autolysosomes.
• Expressed in professional APCs: monocytes/macrophages, dendritic cells, and B cells.
• Post‑translational modifications include glycosylation, disulfide bond formation, and ubiquitination. Keywords: Adaptive immunity, MHC II, Glycoprotein, Signal, Transmembrane.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–227, suggesting the antibody may recognize multiple epitopes of the extracellular domain. Validate in relevant APC lines (e.g., Raji B cells, monocyte-derived dendritic cells) for WB and ELISA.
• For WB, use reducing SDS-PAGE and load 20–30 µg of lysate; the predicted molecular weight is ~30 kDa under reducing conditions. Optimize blocking with 5% BSA.
• For ELISA, coat recombinant protein or cell lysate at 1–2 µg/mL; check cross-reactivity against related HLA-DR beta chains.
• Avoid multiple freeze-thaw cycles and use fresh protease inhibitor cocktails to maintain antigen integrity.
• Pair with an appropriate secondary antibody and consider titrating the primary antibody (e.g., 1:500–1:2000 for WB) to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, offering validated antibodies, antigen pairs, and ancillary reagents for diagnostic development. This anti-HLA-DRB3 polyclonal antibody supports immunoassay research in adaptive immunity and MHC class II biology. Contact CamelBio for bulk supply, custom antibody services, and integrated technical consultation from concept to clinic.