Anti-HLA-DRA Rabbit Polyclonal Antibody for WB, ELISA - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DRA Function and Localization

• HLA-DRA encodes the alpha chain of the MHC class II molecule HLA-DR, which forms a heterodimer with the beta chain (HLA-DRB) to present peptide antigens on the surface of professional antigen-presenting cells (APCs).
• The HLA-DR heterodimer presents extracellular peptide antigens (10–30 amino acids) to CD4+ T cells via the T cell receptor (TCR), thereby orchestrating T-helper cell-mediated adaptive immune responses, including antibody production and macrophage activation.
  Related references: PMID:15265931 PMID:15322540 PMID:17334368
• Peptide binding to MHCII is characterized by a large hydrophobic anchor residue at position 1 of the peptide N-terminus, which is critical for high-affinity interaction with the MHCII molecule.
  Related references: PMID:8145819
• Within the tumor microenvironment, HLA-DRA mediates presentation of tumor-derived peptides, which are primarily generated by tumor-resident APCs via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins.
  Related references: PMID:31495665
• HLA-DRA participates in macroautophagy-dependent presentation of intracellular antigens, a process essential for thymic T cell selection and central immune tolerance.
  Related references: PMID:17182262 PMID:23783831
• Epitope selection for HLA-DR presentation follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigens and 'cut first, bind later' for self-peptides.
  Related references: PMID:25413013
• Subcellularly, the protein localizes to the cell membrane, endoplasmic reticulum, early and late endosomes, lysosomes, and autolysosomes, reflecting its trafficking through the antigen-processing and presentation pathway.
• HLA-DRA is expressed at the protein level in professional APCs such as macrophages, dendritic cells, and B cells, as well as in thymic epithelial cells.
  Related references: PMID:15322540 PMID:23783831 PMID:31495665
• The protein bears structural features including an N-terminal signal peptide, a transmembrane region, disulfide bonds, glycosylation, and ubiquitin conjugation, underscoring its complex regulation and post-translational modification.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 26–216 of human HLA-DRA (extracellular domain). For ELISA, consider using the full-length recombinant protein as a positive control and standard.
• For Western blot, the predicted molecular weight is approximately 29 kDa. Ensure complete protein transfer and use appropriate molecular weight markers; validate with a known positive sample (human, mouse, or rat).
• Cross-reactivity with mouse and rat is reported; however, sequence homology should be verified in silico and the antibody's performance should be titrated and confirmed in the relevant species' tissue or cell lysates.
• This polyclonal antibody is suitable for WB and ELISA; for applications such as immunohistochemistry or flow cytometry, additional validation may be required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD-grade raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for immunology and MHC class II studies. For projects requiring rare target sourcing or custom development, our one-stop supply chain bridges the gap from early research to clinical application, streamlining procurement for your antigen presentation and T cell biology workflows.