Anti-HLA-DRA Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P01903

Core Product Specifications and Parameters

Biological Background: HLA-DRA Function and Localization

• HLA-DRA is the alpha chain of MHC class II, also known as MHC class II antigen DRA, and pairs with HLA-DRB beta chain to form the antigen-presenting complex.
• In complex with HLA-DRB, presents antigenic peptides on professional APCs for CD4+ T cell recognition, guiding T-helper effector functions including antibody-mediated immune response and macrophage activation. Related references: PMID:15265931 PMID:15322540 PMID:17334368
• Typically presents extracellular peptide antigens of 10 to 30 amino acids arising from proteolysis of endocytosed antigens in lysosomes. Related references: PMID:8145819
• In the tumor microenvironment, presents antigenic peptides primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Related references: PMID:31495665
• Presents peptides derived from intracellular proteins trapped in autolysosomes after macroautophagy, a mechanism relevant for T cell selection in the thymus and central immune tolerance. Related references: PMID:17182262 PMID:23783831
• The selection of immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived peptides and 'cut first, bind later' for autoantigens/self-peptides. Related references: PMID:25413013
• The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules. Related references: PMID:8145819
• HLA-DRA localizes to the cell membrane, endoplasmic reticulum membrane, early/late endosome membranes, lysosome membrane, and autolysosome membrane.
• Expressed in professional APCs (macrophages, dendritic cells, B cells) and thymic epithelial cells. Related references: PMID:15322540 PMID:23783831 PMID:31495665
• Glycoprotein with disulfide bonds and a transmembrane helix; undergoes post-translational modifications essential for MHCII stability and function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 100-200 of human HLA-DRA; for analytical validation, consider using recombinant full-length protein or cell lysates expressing native MHC class II complexes.
• For Western blot (WB), a band at approximately 29 kDa is expected under reducing conditions; optimize primary antibody dilution and use appropriate secondary detection reagents.
• For immunohistochemistry (IHC-P), antigen retrieval may be required, and it is advisable to validate the staining pattern in positive control tissues such as lymph node or spleen.
• In ELISA, ensure optimal coating with target antigen and validate using known positive and negative control samples.
• Given that this antibody is raised against a human sequence, confirm cross-reactivity when using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research institutes, and clinical laboratories. With expertise in immunology targets like HLA-DRA, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our global supply network enables efficient procurement of rare-target raw materials and customized solutions to accelerate your assay development from concept to clinic.