Anti-HLA-DQB1 Polyclonal Antibody for WB, IHC-P, ELISA - P01920

Core Product Specifications and Parameters

Biological Background: HLA-DQB1 Function and Localization

• HLA-DQB1 encodes the beta chain of the HLA-DQ class II molecule, which forms a heterodimer with an alpha chain (HLA-DQA1) to present antigens to CD4+ T cells.
• The peptide-binding cleft accommodates antigens of 10–30 residues, primarily derived from exogenous proteins internalized via the endocytic pathway and processed in endosomal/lysosomal compartments.
• Assembly of the MHC class II complex occurs in the endoplasmic reticulum, where three alpha-beta heterodimers associate with an invariant chain trimer (CD74) to form a nonamer.
• Upon trafficking to endosomal compartments, CD74 is sequentially cleaved by cathepsins, leaving a CLIP fragment in the peptide groove; HLA-DM then catalyzes CLIP exchange with high-affinity antigenic peptides.
• Subcellular localization extends across the secretory and endocytic pathways: cell membrane, endoplasmic reticulum membrane, trans-Golgi network membrane, endosome membrane, and lysosome membrane.
• The protein undergoes post-translational modifications including N-linked glycosylation and disulfide bond formation, and contains a signal peptide and a transmembrane helix.
• Beyond professional antigen-presenting cells, aberrant expression in gastrointestinal epithelial cells can contribute to immune responses in organs like the gut.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 33–127 of the mature protein, a region likely exposed in the extracellular domain; this design supports detection in Western blot (WB), immunohistochemistry (IHC-P), and ELISA.
• In WB, a band around 30 kDa is expected, though glycosylation may result in a slightly higher apparent molecular weight; consider using a reducing agent and running appropriate controls.
• For IHC-P, validate the antibody on known HLA-DQB1-positive tissues (e.g., lymph node, tonsil) using standard antigen retrieval protocols; always include a negative control to assess specificity.
• Cross-reactivity is confirmed for human; performance in other species has not been specified—validate in the intended model organism if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-HLA-DQB1 product. Our portfolio covers critical immunology targets, and we offer bulk ancillary reagents and customized sourcing for rare antibody specificities. Whether you need this anti-HLA-DQB1 antibody for human leukocyte antigen typing or autoimmune disease research, CamelBio streamlines procurement with one-stop technical consulting and supply-chain support to accelerate your assay development.