Anti-HLA-DQA1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P01909

Core Product Specifications and Parameters

Biological Background: HLA-DQA1 Function and Localization

• HLA-DQA1 encodes the alpha chain of HLA class II molecule DQ, which pairs with a beta chain (HLA-DQB1) to form a functional MHC class II heterodimer expressed on antigen-presenting cells (APCs).
• The MHC class II molecule binds peptides of 10-30 residues derived from antigens that access the endocytic route, and presents them on the cell surface for recognition by CD4+ T cells, initiating adaptive immune responses.
• Antigen loading occurs in endosomal/lysosomal compartments where the invariant chain (CD74) is sequentially cleaved, leaving the CLIP fragment; HLA-DM facilitates CLIP removal and stabilizes the molecule until high-affinity peptide binds.
• HLA-DQA1 is expressed primarily on professional APCs like dendritic cells, B cells, and macrophages, but also on gastrointestinal epithelial cells, which can act as non-professional APCs.
• Subcellularly, the protein traffics through the endoplasmic reticulum, Golgi, endosomes, and lysosomes, eventually localizing to the cell membrane for peptide presentation.
• Post-translational modifications include glycosylation and a disulfide bond, which are critical for proper folding and function.
• The alias CELIAC1 indicates a genetic association with celiac disease, reflecting its role in autoimmune susceptibility.
• Keywords highlight its involvement in adaptive immunity, membrane targeting, and proteomics identification.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band around 28 kDa; consider potential glycosylation shifts. Validate in lysates from human or mouse cells known to express MHC class II.
• For immunohistochemistry (IHC-P), optimize antigen retrieval conditions and confirm expression in tissue sections rich in APCs (e.g., lymphoid organs) or inflamed gastrointestinal epithelium.
• In ELISA applications, the antibody can be used as a capture or detection reagent; pair with a suitable anti-HLA-DQA1 detection antibody if building a sandwich assay.
• Cross-reactivity with mouse enables translational studies in mouse models, but verify species-specific epitope recognition by sequence alignment.
• This polyclonal antibody, raised against the extracellular domain (aa 24-216), may recognize both native and denatured protein—suitable for multiple assay formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering this HLA-DQA1 polyclonal antibody alongside validated antibody pairs, optimized monoclonal reagents, and bulk ancillary supplies tailored for immunology and autoimmune disease research. Whether you need consistent lot-to-lot performance in diagnostic kits or support in identifying rare-target antibodies, our one-stop platform bridges your development pipeline from concept to clinic.