Anti-HLA-DMB Rabbit Monoclonal Antibody for WB, ELISA - P28068

Core Product Specifications and Parameters

Biological Background: HLA-DMB Function and Localization

• The HLA-DMB protein is the beta chain of the HLA class II histocompatibility antigen DM molecule, also known as MHC class II antigen DMB and RING7 (Really Interesting New Gene 7).
• It plays a critical role in adaptive immunity by catalyzing the release of the class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules, thereby freeing the peptide-binding site for antigenic peptide loading.
• In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO, mediating further control over antigen presentation.
• HLA-DMB is localized to the membranes of late endosomes and lysosomes, the intracellular compartments where MHC class II peptide loading occurs.
• It contains an immunoglobulin domain, a signal peptide, a transmembrane helix, and a disulfide bond, and is post-translationally modified by glycosylation.
• The protein has a calculated molecular weight of approximately 28.9 kDa.
• Keywords associated with HLA-DMB include adaptive immunity, membrane, MHC II, endosome, lysosome, glycoprotein, immunoglobulin domain, and transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human HLA-DMB, likely recognizing epitopes in the N-terminal region; consider this when designing detection or capture assays.
• For Western blotting, the predicted molecular weight is ~29 kDa; validate the antibody in the relevant sample system and optimize electrophoretic and transfer conditions.
• In ELISA, ensure that the coating antigen contains the immunogen sequence or the native protein to confirm specificity, and validate performance using human cell lines or tissue lysates.
• As a transmembrane protein located in endosomal/lysosomal compartments, membrane protein enrichment may improve detection sensitivity in Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with reliable IVD raw materials like this anti-HLA-DMB monoclonal antibody. Specializing in immunology targets, we provide validated antibody pairs, optimized recombinant antibodies, bulk ancillary reagents, and hard-to-find raw material sourcing to support every stage of assay development—from research to regulatory approval.