Anti-HLA B7 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P01889

Core Product Specifications and Parameters

Biological Background: HLA-B (HLA B7) Function and Localization

• HLA-B is a classical MHC class I heavy chain that pairs with β2-microglobulin to present peptide antigens (8-13 residues) to CD8+ T cells, critical for adaptive immune surveillance against viruses and tumors (PubMed:23209413, PubMed:25808313, PubMed:29531227, PubMed:24600035, PubMed:9620674). Self-peptide presentation also occurs but usually induces tolerance (PubMed:18991276, PubMed:7743181). Related references: PMID:23209413 PMID:25808313 PMID:29531227 PMID:24600035 PMID:9620674 PMID:18991276 PMID:7743181

• HLA-B is extremely polymorphic with numerous alleles (e.g., B07:02, B27:05) that differ in their peptide-binding motifs, influencing individual immune responses and disease associations. For example, B*07:02 favors Pro at P2 and Leu at the C-terminus (PubMed:25808313, PubMed:29531227, PubMed:7743181). Related references: PMID:25808313 PMID:29531227 PMID:7743181

• Subcellular localization is primarily at the cell membrane and endoplasmic reticulum membrane, consistent with its role in peptide loading in the ER and transport to the cell surface.

• It is a glycoprotein with a conserved disulfide bond, and phosphorylation may occur (as indicated by keywords: Glycoprotein, Disulfide bond, Phosphoprotein). Post-translational modifications can influence stability and antigen presentation.

• Specific alleles present viral epitopes: B07:02 presents HIV-1 and SARS-CoV-2 epitopes (PubMed:25808313, PubMed:32887977); B08:01 presents EBV epitopes; B*27:05 presents HCV, HIV, influenza, and SARS-CoV-2 epitopes and confers viral protection (PubMed:15113903, PubMed:18385228, PubMed:19139562, PubMed:32887977). Related references: PMID:25808313 PMID:32887977 PMID:15113903 PMID:18385228 PMID:19139562

• HLA-B also interacts with NK cell inhibitory receptors like KIR3DL1, modulating innate immunity in a peptide-dependent manner (PubMed:15657948, PubMed:8879234). Related references: PMID:15657948 PMID:8879234

Experimental Guidance and Technical Tips

• The N-terminal peptide immunogen (aa 1-100) suggests the antibody may recognize linear epitopes in both denaturing and native conformations; consider testing under reducing and non-reducing conditions for Western blot.
• In Western blot, expect a band around 40–45 kDa, depending on glycosylation; include a human lymphocyte lysate as a positive control to confirm specificity.
• For immunofluorescence/ICC, verify plasma membrane localization typical of MHC I; optimize fixation/permeabilization to preserve epitope accessibility.
• For ELISA, validate the antibody in your sample type, as soluble HLA-B can be detected in serum and culture supernatants; consider pairing with a well-characterized detection antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and IVD developers with a comprehensive supply chain for raw materials, including validated monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents. This anti-HLA B7 rabbit monoclonal antibody supports immunoassay development for immune monitoring, transplantation immunology, and viral infection studies. Beyond this product, we source specialized HLA and MHC class I reagents to accelerate your assay development from concept to clinic.