Anti-HLA-B Rabbit Polyclonal Antibody for WB - P01889

Core Product Specifications and Parameters

Biological Background: HLA-B Function and Localization

• HLA-B, also known as human leukocyte antigen B, is a classical major histocompatibility complex class I (MHC I) heavy chain that assembles with β2-microglobulin (B2M) to form a functional antigen-presenting complex on the cell surface.
• It plays a central role in adaptive immunity by presenting primarily viral and tumor-derived peptides of 8-13 amino acids to αβ T cell receptors (TCR) on CD8+ T cells, triggering antigen-specific cytotoxic responses. May also display self-peptides under tolerogenic conditions. Related references: PMID:23209413 | PMID:25808313 | PMID:29531227 | PMID:18991276 | PMID:7743181 | PMID:24600035 | PMID:9620674
• HLA-B is highly polymorphic with over 9000 alleles; different alleles exhibit distinct peptide-binding motifs. For example, allele B07:02 preferentially binds peptides with Pro at position 2 and a C-terminal Leu, while B27:05 requires an Arg at position 2. Related references: PMID:7743181 | PMID:29531227 | PMID:1922338
• The mature protein localizes predominantly to the cell membrane and endoplasmic reticulum membrane, consistent with its role in peptide loading and surface presentation.
• Post-translational modifications include a cleaved signal peptide, N-linked glycosylation, a conserved disulfide bond in the α2 domain, and phosphorylation. The UniProt record also annotates the protein as a transmembrane glycoprotein involved in host‑virus interactions.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic peptide spanning amino acids 263–362 of human HLA-B, a region located in the extracellular domain. This design supports detection of the mature protein.
• For Western blot, prepare samples under reducing conditions and separate on SDS-PAGE; expect a band near 40 kDa. Validate performance in your sample matrix and loading amounts.
• Given the high polymorphism of HLA-B, cross‑reactivity with different alleles should be assessed in the relevant cell lines or tissues.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials for immunology researchers and diagnostic developers. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support HLA‑B‑related immunoassay and infectious disease research. Partner with us for efficient sourcing from concept to clinic.