Anti-HK3 Polyclonal Antibody for WB, IF/ICC, ELISA - P52790

Core Product Specifications and Parameters

Biological Background: HK3 Function and Localization

• Hexokinase-3 (HK3), also designated hexokinase type III, hexokinase-C, HXK3, or HKIII, is an allosteric enzyme belonging to the hexokinase family.
• HK3 catalyzes the ATP-dependent phosphorylation of hexoses (e.g., D-glucose and D-fructose) to hexose 6-phosphate, mediating the first committed step of glycolysis. Related references: PMID:8717435
• The protein contains nucleotide-binding and ATP-binding domains, classifying it as a transferase and kinase.
• HK3 is a repeat-containing protein, and its three-dimensional structure has been determined, offering insight into its allosteric regulation.
• Identified through large-scale proteomics as a reference proteome component, HK3 is part of the comprehensive catalog of human proteins.
• With a molecular mass of approximately 99 kDa, HK3 plays a critical role in cellular glucose metabolism and is a potential target in metabolic and cancer research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–270 of human HK3 (NP_002106.2), an N‑terminal fragment encompassing key regulatory regions. In western blotting, expect a band near 99 kDa in human, mouse, or rat cell lysates.
• For immunofluorescence/immunocytochemistry, validate staining in relevant cell lines from human, mouse, or rat. Co‑stain with organelle markers if subcellular localization is being investigated, as endogenous HK3 distribution is not fully characterized.
• In ELISA applications, the antibody may be employed as a capture or detection reagent; titrate carefully and confirm performance using recombinant HK3 protein or known positive samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a comprehensive sourcing platform for IVD raw materials. For glycolysis and cancer metabolism studies, we offer validated antibodies like this anti-HK3 polyclonal antibody, along with matched antigen reagents, monoclonal alternatives, and ancillary bulk materials, all supported by expert technical consulting from concept to clinic.