Anti-HJURP Rabbit Polyclonal Antibody for WB and ELISA - Q8NCD3

Core Product Specifications and Parameters

Biological Background: HJURP Function and Localization

• HJURP (Holliday junction recognition protein) is a centromeric protein acting as a specific chaperone for the histone H3-like variant CENPA, crucial for CENPA incorporation into nucleosomes at replicated centromeres.
• It prevents premature CENPA-H4 tetramerization and DNA binding, ensuring proper centromere assembly and maintenance.
• The protein directly binds Holliday junctions, suggesting a role in DNA repair or recombination processes at the centromere.
• HJURP localizes predominantly to the nucleus, specifically accumulating in the nucleolus and at centromeres during cell division.
• Tissue expression analysis reveals high levels in thymus and testis, with lower expression in placenta, small intestine, liver, skeletal muscle, colon, and bone marrow; it is significantly overexpressed in many lung cancer samples compared to normal lung. Related references: PMID:17256767 PMID:17823411
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond formation), which may regulate its chaperone function and cell cycle-dependent localization.
• Alternative splicing generates multiple isoforms, and the protein is classified under keywords highlighting its roles in cell cycle, centromere, chromosome, and DNA binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-190), so the antibody is likely to recognize full-length and possibly N-terminal isoforms; consider verifying isoform specificity in your target samples.
• For Western blot, the predicted molecular weight is 84 kDa; use appropriate gel percentages and transfer conditions for high-molecular-weight proteins.
• The antibody is validated for ELISA; for chromatin immunoprecipitation (ChIP) or immunofluorescence, optimize conditions as cross-reactivity to native complexes may vary.
• Cross-reactivity with mouse and rat is reported, making it suitable for rodent model studies; however, sequence alignment across species is advised to confirm epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized supplier of raw materials for the IVD industry, providing validated antibodies, bulk ancillary reagents, and rare target sourcing to diagnostic manufacturers and research labs worldwide. This anti-HJURP polyclonal antibody supports research into centromere biology and chromatin dynamics, areas critical for cancer biomarker development. Explore our extensive portfolio for antibody pairs, recombinant proteins, and customized solutions to accelerate your assay development from concept to clinic.