Anti-Histone H4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P62805

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is one of the core histone proteins that form the nucleosome, the fundamental repeating unit of chromatin.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability by controlling DNA accessibility.
• The human genome contains multiple histone H4 gene copies (e.g., H4C1, H4C2, H4C3, etc.) that encode an identical histone H4 protein.
• Histone H4 is localized in the nucleus and associated with chromosomes.
• It undergoes extensive post-translational modifications, including acetylation, methylation, phosphorylation, citrullination, hydroxylation, and ubiquitination, which constitute the "histone code" and regulate chromatin dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the conserved histone fold domain (aa 24-103), suggesting potential recognition of both unmodified and some modified forms of Histone H4; however, detection of specific post-translational modifications should be validated experimentally.
• For Western blot, Histone H4 migrates around 11 kDa; consider using 15% SDS-PAGE gels or gradient gels for optimal resolution of low-molecular-weight proteins.
• In IHC-P, expect nuclear staining; antigen retrieval conditions (e.g., citrate buffer pH 6.0) should be optimized for the specific tissue type.
• Western blot and ELISA applications can be used to assess antibody reactivity across different species or to quantify Histone H4 levels; always include appropriate controls.
• Due to high sequence conservation among species, cross-reactivity with a wide range of organisms is predicted; verify performance in your target species or sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner that connects diagnostic manufacturers and research teams with high-performance reagents. For chromatin and epigenetics research, we offer validated antibody pairs, optimised monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our Histone H4 monoclonal antibody supports your assay development and biomolecular studies, backed by reliable supply and technical expertise.