Anti-Histone H4 Polyclonal Antibody for WB - P62805

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Histone H4 is encoded by a large gene family (H4C1-H4C16) that produces an identical protein, ensuring robust expression during S-phase.
• As a core histone, it assembles into nucleosomes to compact DNA into chromatin, essential for genome organization and integrity.
• Histone H4 regulates transcription, replication, and repair by influencing DNA accessibility, central to epigenetic control.
• Extensive post-translational modifications (acetylation, methylation, phosphorylation, etc.) create a histone code that modulates chromatin dynamics.
• Localized exclusively in the nucleus and on chromosomes, it is fundamental to chromosomal architecture.
• Mutations in H4 genes are linked to developmental disorders like intellectual disability, highlighting its biological importance.

Experimental Guidance and Technical Tips

• Due to the high sequence conservation of Histone H4 across species, the antibody may detect a broad range of mammalian and other vertebrate samples; validate in your specific model.
• The immunogen corresponds to the N-terminal region (aa 1-100), which is subject to numerous post-translational modifications; consider that heavy modifications in this region might affect antibody binding.
• For Western blot, the predicted molecular weight is 11 kDa; use appropriate gel percentage and transfer conditions for low molecular weight proteins.
• For IF/ICC, ensure proper nuclear permeabilization to access the chromatin-bound target.
• ELISA may be used to quantify histone modifications or total H4 levels; validate with appropriate peptide controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to validated IVD raw materials, supporting the entire development pipeline. For Histone H4-related chromatin and epigenetics research, we offer optimized polyclonal antibodies, recombinant proteins, and ancillary reagents in bulk. Our specialized sourcing network also assists in procurement of rare-target materials to accelerate your assay development.