Anti-Histone H4 Polyclonal Antibody for WB, ELISA - P62805

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

• Core histones, including H4, form the nucleosome core particle together with H2A, H2B, and H3, constituting the fundamental repeating unit of chromatin.
• Histone H4 wraps approximately 147 base pairs of DNA around the histone octamer, compacting the genome and regulating access to genetic information.
• In humans, Histone H4 is encoded by a multi-gene family (H4C1 to H4C16) that yields identical protein sequences, ensuring sufficient histone supply for DNA packaging.
• It plays essential roles in transcription regulation, DNA repair, DNA replication, and chromosomal stability by controlling chromatin structure.
• Subcellularly, Histone H4 is localized to the nucleus and chromosomes, where it participates in higher-order chromatin organization.
• Undergoes extensive post-translational modifications—including acetylation, methylation, phosphorylation, citrullination, and ubiquitination—that form the 'histone code' and influence chromatin dynamics.
• Keywords associated with Histone H4 include chromosomal rearrangement and disease variants linked to intellectual disability, highlighting its importance in development.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 (N-terminal region), which is highly conserved; therefore, broad cross-reactivity across species is expected. Consider validating specificity in your target species.
• For Western blot, nuclear-enriched lysates or acid-extracted histone preparations are recommended to detect the 11 kDa band. The antibody is also suitable for ELISA; optimize coating conditions using purified histones or peptides.
• Given the high evolutionary conservation of histone sequences, this antibody may recognize multiple H4 variants; always confirm identity by molecular weight and fractionation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for IVD development. From nucleosome targets like Histone H4 to rare chromatin-related raw materials, we provide one-stop procurement support—streamlining your transition from concept to clinic.