Anti-Histone H3.3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA, ChIP, ChIP-seq - P84243

Core Product Specifications and Parameters

Biological Background: Histone H3.3 Function and Localization

• Variant histone H3 that replaces conventional H3 in nucleosomes at actively transcribed genes, serving as the predominant H3 form in non-dividing cells and depositing independently of DNA synthesis
• Core component of nucleosomes, wrapping and compacting DNA into chromatin to restrict template accessibility; thereby central in transcription regulation, DNA repair, DNA replication, and chromosomal stability
• Subject to a complex array of post-translational modifications (acetylations, methylations, phosphorylations, etc.) that constitute the histone code, regulating chromatin dynamics and gene expression
• Localizes exclusively to the nucleus and chromosomes, enriched at regions of nucleosomal displacement throughout gene bodies, marking transcriptionally active chromatin
• Encoded by two genes, H3-3A (H3F3A) and H3-3B (H3F3B), producing identical protein products; distinct from canonical replication-dependent H3 variants
• Associated with disease variants linked to intellectual disability and developmental disorders, underscoring its critical epigenetic role
• Highly conserved across vertebrates, with a predicted molecular weight of approximately 15,328 Da, though often observed migrating around 15–17 kDa in SDS-PAGE due to PTMs

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 57–136 of human Histone H3.3, a region involved in nucleosome interactions; this epitope may be conformationally sensitive—consider testing under native and denaturing conditions.
• For WB, a band around 15 kDa is expected; validate in your specific sample system and use appropriate loading controls (e.g., total H3 or housekeeping proteins).
• Given reported cross-reactivity with Human, Mouse, Rat, and a wide range of other species, pilot testing in non-standard models is advisable.
• In ChIP and ChIP-seq, optimize crosslinking and shearing conditions; the antibody’s performance should be verified with positive (active gene) and negative (silent locus) control regions.
• For IHC-P, antigen retrieval and antibody dilution should be empirically determined; nuclear staining pattern is expected.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, from concept to clinic. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for chromatin and epigenetic research, including rare targets like histone variants. Our global network ensures reliable, scalable supply tailored to diagnostic manufacturers and laboratories.