Anti-Histone H3 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA, ChIP - Q16695 / P68431

Core Product Specifications and Parameters

Biological Background: Histone H3 Function and Localization

• Histone H3.1t (also known as H3.4, H3/g) is a core component of the nucleosome, which wraps and compacts DNA into chromatin, limiting DNA accessibility to the cellular machineries that require DNA as a template. This plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• Nucleosomes consist of two copies of each core histone (H2A, H2B, H3, H4). Histone H3 is integral to the histone code hypothesis, where its diverse post-translational modifications (acetylation, methylation, phosphorylation, etc.) govern chromatin structure and gene expression.
• Encoded by the H3-4 (HIST3H3) gene, this testis-specific histone variant is a member of the replication-dependent histone H3 family, with expression restricted primarily to testicular cells.
• The protein localizes strictly to the nucleus and is associated with chromosomes, consistent with its role in chromatin organization.
• Key post-translational modifications include acetylation, methylation, phosphorylation, citrullination, ADP-ribosylation, and ubiquitination, reflecting its dynamic regulation during the cell cycle and development.
• It possesses DNA-binding activity, contributing to the formation of higher-order chromatin structures and epigenetic inheritance mechanisms.
• With a calculated molecular weight of approximately 15.5 kDa (UniProt) and observed at 15 kDa, it migrates as a typical core histone in SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen was designed against a peptide spanning amino acids 50-136 of human HIST3H3, a region likely exposed in the nucleosome context; consider using this antibody in applications such as WB, IHC-P, IP, ELISA, and ChIP as indicated.
• Given the wide predicted cross-reactivity, validate the antibody in your specific species and tissue of interest, particularly for non-human samples.
• Histone H3 is highly conserved across species; consider potential cross-reaction with other H3 variants if precise variant discrimination is required.
• Optimize antibody concentration by titration for each application, as performance may vary between assays. For ChIP, ensure proper crosslinking and shearing conditions are employed.
• For western blotting, total histone extracts or acid-extracted histones may provide cleaner signals due to the basic nature of the protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic development from concept to clinic. This anti-Histone H3 antibody serves as a valuable tool for chromatin and epigenetic research, enabling robust assay development for applications like western blotting and immunohistochemistry. Whether you need custom antibody generation, bulk supply, or specialized technical support, CamelBio bridges your research and diagnostic needs with efficient, one-stop solutions.