Anti-Histone H2B (testis specific) Rabbit Polyclonal Antibody for WB, ELISA - Q96A08

Core Product Specifications and Parameters

Biological Background: Histone H2B type 1-A Function and Localization

• Histone H2B type 1-A (H2BC1/HIST1H2BA/TSH2B) is a testis-specific variant histone that entirely replaces canonical H2B prior to the nucleosome-to-protamine transition during spermatogenesis (By similarity).
• It acts as a nucleosome-dissociating factor, creating a more dynamic chromatin state that facilitates the large-scale exchange of histones with protamines in male germ cells (By similarity).
• As a core component of nucleosomes, it help wrap and compact DNA into chromatin, thereby regulating DNA accessibility for processes such as transcription, DNA repair, replication, and chromosomal stability (By similarity).
• This histone is subject to various post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitination, which contribute to the histone code and chromatin remodeling (By similarity).
• Its subcellular localization is strictly nuclear, associated with chromosomes, consistent with its role in chromatin structure.
• While predominantly expressed in testis and mature sperm, it is also detected in certain fat cells, where its specific function remains unclear. Related references: PMID:21249133

Experimental Guidance and Technical Tips

• The immunogen is a recombinant full-length human Histone H2B (testis specific) protein; the resulting polyclonal antibody may recognize both native and denatured forms. Empirical testing of dilutions for specific applications is recommended.
• A predicted molecular weight of ~14 kDa can be used as a reference in WB experiments; verify band specificity against appropriate positive and negative controls.
• For ELISA applications, consider pairing with a suitable detection antibody and validate assay conditions using recombinant protein or cell lysates.
• Cross-reactivity with mouse and rat orthologs enables comparative studies, but independent validation in each species’ sample system is advised to confirm reactivity and specificity.

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CamelBio provides diagnostic manufacturers and research institutes with streamlined access to high-quality IVD raw materials like this testis-specific histone H2B polyclonal antibody, ideal for chromatin and spermatogenesis studies. We offer one-stop procurement solutions including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Let CamelBio bridge your supply needs from concept to clinic.