Anti-Histone H2B (testis specific) Monoclonal Antibody for WB, IP, IHC - Q96A08

Core Product Specifications and Parameters

Biological Background: Histone H2B type 1-A Function and Localization

• Histone H2B type 1-A (H2BC1, also known as TSH2B.1/hTSH2B) is a testis-specific variant histone that replaces classical histone H2B during spermatogenesis.
• It specifically directs the transformation of dissociating nucleosomes to protamine in male germ cells, acting as a nucleosome dissociating factor to create dynamic chromatin facilitating large-scale histone exchange (By similarity).
• As a core component of the nucleosome, it wraps and compacts DNA into chromatin, playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability (By similarity).
• DNA accessibility is regulated through a complex set of post-translational modifications (histone code) and nucleosome remodeling (By similarity).
• Histone H2B type 1-A is also expressed in some fat cells; its function and specific post-translational modifications in these cells remain unclear (PubMed:21249133). Related references: PMID:21249133
• Subcellular localization is restricted to the nucleus and chromosomes.
• Known post-translational modifications include acetylation, methylation, phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• While predominantly expressed in testis and mature sperm (at protein level), it is also detected in certain fat cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human Histone H2B type 1-A; therefore, the antibody likely detects an epitope in this region, which is conserved across the predicted cross-reactive species.
• For Western blot, expect a band at ~14 kDa; validate in a relevant sample system such as human testis lysate or other tissues expressing this variant.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), consider testis tissue sections where expression is highest; optimization of antigen retrieval may be required.
• For immunoprecipitation (IP), use a nuclear extract from testis or cells expressing the target; confirm interactions with protamine exchange factors if relevant.
• As this histone variant is also found in some fat cells, researchers may detect bands or staining in adipose tissue or adipocyte cultures; consider using appropriate controls to verify specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality antibodies, antigens, and ancillary reagents. This testis-specific Histone H2B antibody supports studies in chromatin dynamics and male fertility research. Leverage CamelBio’s supply network for validated antibody pairs, bulk reagent orders, and custom sourcing of rare-target raw materials to accelerate assay development.