Anti-Histone H2B Rabbit Polyclonal Antibody for WB, ELISA - P62807 / Q16778

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

• Core component of nucleosomes, responsible for wrapping and compacting DNA into chromatin, thereby regulating DNA accessibility for transcription, DNA repair, DNA replication, and chromosomal stability.
• Participates in the histone code, a complex set of post-translational modifications (including acetylation, methylation, phosphorylation, ADP-ribosylation, hydroxylation, and ubiquitination) that modulate nucleosome structure and function.
• Exhibits broad-spectrum antibacterial activity and may contribute to the antimicrobial barrier of the colonic epithelium as well as the bactericidal action of amniotic fluid.
• Localizes predominantly in the nucleus and associates with chromosomes.
• Encoded by multiple gene family members (e.g., H2BC4, H2BC6, H2BC7, H2BC8, H2BC10), producing highly similar histone H2B isoforms; this antibody targets a conserved N-terminal region, likely recognizing multiple variants.
• Characterized as a DNA-binding, antimicrobial, and extensively modified protein (direct protein sequencing, reference proteome), underscoring its critical roles in both nuclear architecture and innate immunity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human Histone H2B, corresponding to a highly conserved region; thus, the antibody may exhibit broad cross-reactivity across species beyond those listed, as indicated by the “Wide Range Predicted” designation.
• For Western blot, a single band around 14 kDa is expected. Consider optimizing dilution factors (e.g., 1:500–1:2000) using a positive control such as HeLa or HEK293 nuclear extract.
• In ELISA, this antibody can serve as a capture or detection reagent; validate with recombinant Histone H2B or nucleosome preparations in the relevant sample matrix.
• Due to the high conservation of histone sequences, cross-reactivity with other histone family members cannot be ruled out; confirm specificity using isoform-specific controls or knockout/knockdown models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories as a comprehensive supplier of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-Histone H2B antibody is part of our growing portfolio for chromatin and epigenetics research, complementing our capabilities in nucleosome and histone modification studies. We offer end-to-end procurement solutions to accelerate your assay development from concept to clinic.