Anti-Histone H2B Rabbit Monoclonal Antibody for WB - P62807 / Q16778

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

• Histone H2B is a core component of the nucleosome, which wraps and compacts DNA into chromatin, thereby regulating transcription, DNA repair, replication, and chromosomal stability.
• DNA accessibility is modulated through a complex set of post-translational modifications (the histone code) and nucleosome remodeling, with H2B being subject to acetylation, methylation, phosphorylation, ubiquitination, and other PTMs.
• The immunogen targets the canonical H2B type 1 isoforms (C/E/F/G/I), encoded by a cluster of genes including H2BC4, H2BC6, H2BC7, H2BC8, and H2BC10.
• In addition to its nuclear role, H2B exhibits broad antibacterial activity and may contribute to the antimicrobial barrier of the colonic epithelium and the bactericidal activity of amniotic fluid.
• Subcellular localization is strictly nuclear, associated with chromosomes.
• Key structural features and functional regulation involve an isopeptide bond, hydroxylation, and ADP-ribosylation, among other modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human Histone H2B (aa 1–126); for Western blotting, expect a band at approximately 14 kDa. Consider using histone extraction protocols and validate detection in relevant nuclear lysates.
• For IHC-P, suitable antigen retrieval and permeabilization steps may be required; validate staining patterns against known subcellular localization (nucleus/chromatin).
• In ChIP, consider cross-linking optimization and appropriate controls (e.g., histone H3 or IgG) to verify enrichment at target genomic loci.
• ELISA can be used for quantitative detection; titrate antibody and validate against recombinant H2B or nucleosome substrates.
• Cross-reactivity with mouse and rat has been reported; verify suitability for your specific sample type and application by performing appropriate species-specific controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This Histone H2B rabbit monoclonal antibody is ideal for chromatin biology and epigenetics-focused diagnostic assay development. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and expert sourcing of rare-target raw materials.