Anti-Histone H2B Polyclonal Antibody for WB, IHC-P, ELISA - P62807 / Q16778

Core Product Specifications and Parameters

Biological Background: Histone H2B Function and Localization

• Histone H2B is a core component of the nucleosome, playing a central role in compacting DNA into chromatin and regulating DNA accessibility for transcription, repair, replication, and chromosomal stability.
• DNA accessibility is further modulated by a complex set of post-translational modifications on histones, collectively termed the histone code, and by nucleosome remodeling complexes.
• Histone H2B exhibits broad antibacterial activity and may contribute to the antimicrobial barrier of the colonic epithelium as well as the bactericidal properties of amniotic fluid.
• Subcellular localization is predominantly nuclear, associated with chromosomes.
• The protein undergoes a variety of post-translational modifications including acetylation, methylation, phosphorylation, ubiquitination, ADP-ribosylation, hydroxylation, and glycosylation.
• Multiple genes encode histone H2B variants; this antibody recognizes products of several H2BC genes (e.g., H2BC4, H2BC6, H2BC7, H2BC8, H2BC10) corresponding to SWISS P62807 and Q16778, with a predicted molecular weight of approximately 14 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human Histone H2B, which is highly conserved across species; broad cross-reactivity with mouse, rat, and other organisms is expected. Validate in the target species' relevant sample system.
• For Western blotting, a band around 14 kDa is anticipated; however, extensive post-translational modifications may cause migration shifts. Use appropriate low-molecular-weight markers and confirm specificity with controls.
• In immunohistochemical (paraffin) applications, antigen retrieval may be necessary; optimize retrieval conditions based on tissue type and fixation.
• As a polyclonal antibody, lot-to-lot variability may occur; consider independent validation for critical experiments and when switching batches.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to essential IVD raw materials and technical expertise. This anti-Histone H2B polyclonal antibody supports chromatin research and epigenetic assay development, and is part of our broad portfolio of validated antibodies for nucleosome targets. Whether you require validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare-target raw materials, our streamlined supply network helps accelerate your project from concept to clinic.