Anti-Histone H2A.Z Monoclonal Antibody for WB, IP, ELISA, ChIP - P0C0S5

Core Product Specifications and Parameters

Biological Background: Histone H2A.Z Function and Localization

• Histone H2A.Z is a variant histone replacing conventional H2A in a subset of nucleosomes, core components of chromatin that package DNA and regulate accessibility.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability by modulating chromatin structure.
• H2A.Z is involved in the formation of constitutive heterochromatin and may be required for chromosome segregation during cell division.
• Like other histones, H2A.Z is subject to a complex set of post-translational modifications, including acetylation, methylation, and ubiquitination, which contribute to the histone code.
• The protein localizes to the nucleus and is integrated into chromosomes.
• H2A.Z is encoded by the H2AZ1 gene (also known as H2AFZ, H2AZ) in humans.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 1-100) of human Histone H2A.Z, which may be well-suited for detecting the protein in various applications.
• For Western blot (WB), consider validating the antibody in knockout (KO) samples to confirm specificity, as indicated by the product validation.
• In chromatin immunoprecipitation (ChIP), it is advisable to optimize crosslinking and antibody concentration, as histone targets may require careful handling.
• For immunoprecipitation (IP) and ELISA, standard protocols can be employed, but pilot experiments are recommended to determine optimal working dilutions.

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