Anti-Histone H2AX Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P16104

Core Product Specifications and Parameters

Biological Background: Histone H2AX Function and Localization

• Histone H2AX is a variant of the core histone H2A that replaces conventional H2A in a subset of nucleosomes.
• It plays a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability by controlling DNA accessibility.
• Required for checkpoint-mediated cell cycle arrest in response to low doses of ionizing radiation and for efficient repair of DNA double-strand breaks (DSBs) when phosphorylated at its C-terminal tail.
• Localizes to the nucleus and chromatin; rapidly recruited to sites of DNA damage forming γ-H2AX foci.
• Subject to diverse post-translational modifications including acetylation, phosphorylation, and ubiquitination, contributing to the histone code.
• Involved in host-virus interactions and meiosis, highlighting its diverse biological functions.
• Also known as H2A.X, H2A/X, and H2AFX, encoded by the H2AX gene on human chromosome 11.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 50-143) targets a highly conserved region, so broad species cross-reactivity is expected; always validate in your sample system.
• For WB, expect a band at ~15 kDa; use nuclear extracts or whole cell lysates from cells treated with DNA damaging agents (e.g., ionizing radiation, etoposide) to assess phospho-specific detection.
• For IHC-P and IF/ICC, optimize antigen retrieval and nuclear staining; positive controls include tissues or cells exposed to DNA damage.
• For ELISA, consider using a recombinant H2AX protein or peptide coating and perform competitive binding assays to confirm specificity.
• Titration and protocol optimization are essential for each application; start with the recommended dilutions and adjust as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, technical services, and consulting, supporting your projects from concept to clinic. This anti-Histone H2AX antibody, relevant for DNA damage response and chromatin biology studies, is part of our broad antibody collection; we also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your diagnostic development.