Anti-Histone H2AX Monoclonal Antibody for IHC-P, ChIP, ELISA - P16104

Core Product Specifications and Parameters

Biological Background: Histone H2AX Function and Localization

• Histone H2AX is a variant of the canonical histone H2A, substituting for H2A in a subset of nucleosomes.
• Nucleosomes, composed of histone octamers around which DNA wraps, compact the genome and regulate DNA accessibility for transcription, replication, and repair.
• H2AX plays a critical role in the DNA damage response: upon double-strand breaks, its C-terminal serine (Ser139) is rapidly phosphorylated to form γ-H2AX, marking damage sites.
• This phosphorylation is required for efficient DNA double-strand break repair and for checkpoint-mediated arrest of the cell cycle following ionizing radiation.
• Located in the nucleus and on chromosomes, H2AX participates in maintaining genomic stability.
• Post-translational modifications include acetylation, phosphorylation, ubiquitination, and sumoylation, contributing to the histone code and chromatin dynamics.
• The protein is also involved in meiotic recombination and host-virus interactions, as indicated by functional annotations.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminus (aa 1–100) of human Histone H2AX; consider using blocking peptides for specificity controls in your assays.
• For ChIP, cross-linking protocols are recommended due to the tight association of histones with DNA; validate antibody performance in your cell type and chromatin shearing conditions.
• The listed applications (IHC-P, ChIP, ELISA) are based on manufacturer data; optimize antibody dilution and incubation conditions for your specific experimental system.
• With predicted wide cross-reactivity, verify expected target molecular weight (~15 kDa) and species-specific reactivity via Western blot if applied to new species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with seamless access to high-quality IVD raw materials, including this Histone H2AX monoclonal antibody for chromatin and DNA damage studies. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your assay development. For hard-to-find targets, our sourcing expertise connects you with stable supply chains from concept to clinic.