Anti-Histone H2AFV Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q71UI9

Core Product Specifications and Parameters

Biological Background: Histone H2AFV Function and Localization

• Histone H2A.V (also known as H2A.Z-2, H2AFV, H2AV) is a variant histone H2A that replaces canonical H2A in a subset of nucleosomes, contributing to chromatin structural and functional diversity.
• It is encoded by the H2AZ2 gene in humans and belongs to the H2A.Z histone family, which plays key roles in gene regulation and genome stability.
• H2A.V-containing nucleosomes are involved in transcription regulation, DNA repair, DNA replication, and maintenance of chromosomal stability.
• The protein may participate in constitutive heterochromatin formation and is potentially required for proper chromosome segregation during cell division.
• Subcellular localization is nuclear and chromosome-associated, consistent with its role in nucleosome assembly.
• The protein undergoes post-translational modifications including acetylation and ubiquitination, which modulate chromatin dynamics.
• UniProt keywords highlight its involvement in nucleosome core, DNA-binding, alternative splicing, and ubiquitin conjugation pathways.

Experimental Guidance and Technical Tips

• For histone enrichment, consider acid extraction protocols to improve detection of chromatin-bound H2A.V in western blotting.
• The predicted molecular weight of H2A.V is ~13.5 kDa (as calculated from UniProt) with an observed band around 13 kDa; use appropriate low-molecular-weight markers and high-percentage SDS-PAGE gels for optimal resolution.
• In IHC-P and IF/ICC, anticipate nuclear staining; validate antibody specificity using knockout or knockdown controls in your cell/tissue system.
• The immunogen covers the full N-terminal sequence (aa 1-128), which is highly conserved across human, mouse, and rat, supporting cross-reactivity as indicated; perform individual validation for your specific application conditions.
• For ELISA, ensure that the recombinant protein used for coating matches the immunogen format to obtain consistent binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and IVD developers with one-stop access to high-quality raw materials, including validated polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. This anti-Histone H2AFV antibody supports research into chromatin biology and epigenetic regulation, areas critical for oncology, developmental biology, and biomarker discovery. Through our integrated sourcing platform, we help you secure rare-target antibodies, bulk reagents, and customized technical solutions to streamline your assay development from concept to clinic.