Anti-Histone H2A Monoclonal Antibody for WB, IHC-P, ELISA - P04908/P0C0S8

Core Product Specifications and Parameters

Biological Background: Histone H2A Function and Localization

• Histone H2A type 1-B/E (also known as Histone H2A.2, H2A/a, H2A/m) is encoded by the H2AC4 and H2AC8 genes and is a core component of the nucleosome.
• As a nucleosome component, it wraps and compacts DNA into chromatin, restricting DNA accessibility and playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
• The protein localizes to the nucleus and chromosomes.
• It undergoes various post-translational modifications, including acetylation, citrullination, hydroxylation, methylation, phosphorylation, and ubiquitination, which contribute to the histone code and nucleosome remodeling.
• Keywords associated with Histone H2A include DNA-binding, nucleosome core, isopeptide bond, and Ubl conjugation.
• Its alternative names include H2A.1, H2AFA, and HIST1H2AE, reflecting historical isoform designations.
• The predicted molecular weight is approximately 14 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1–100) of human Histone H2A (P04908), a highly conserved region supporting broad species cross-reactivity.
• For western blotting (WB), detection of a band at ~14 kDa is expected; consider using an appropriate histone loading control (e.g., total histone H3) and validate with relevant cell or tissue lysates.
• In immunohistochemistry (IHC‑P), optimal antigen retrieval conditions and antibody dilution should be empirically determined for each sample type.
• For ELISA, specificity can be assessed using the immunogen peptide or recombinant Histone H2A protein; include appropriate negative controls.
• Due to the high conservation of histones, cross-reactivity with other species is predicted but should be confirmed in the target experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one‑stop access to IVD raw materials and technical support for diagnostic manufacturers and research laboratories. Our portfolio includes validated monoclonal antibodies against histone targets, supporting chromatin biology and epigenetics research. We also offer bulk ancillary reagents and can assist with sourcing rare target raw materials to accelerate your assay development.