Anti-Histone H1t Polyclonal Antibody for WB, IHC-P, ELISA - P22492

Core Product Specifications and Parameters

Biological Background: Histone H1t Function and Localization

• Histone H1t, also known as testicular H1 histone, is a testis-specific linker histone that binds to nucleosomes and facilitates higher-order chromatin compaction (Probable).
• Unlike other H1 subtypes, H1t forms less compacted chromatin, which is thought to promote homologous recombination during meiosis (PubMed:26757249). Related references: PMID:26757249
• Encoded by the H1-6 gene (formerly HIST1H1T), H1t is classified among the replication-independent histone H1 variants that exhibit tissue-specific expression patterns.
• Subcellularly, H1t localizes to the nucleus and is associated with chromosomes, consistent with its role as a DNA-binding structural protein.
• H1t undergoes multiple post-translational modifications including acetylation, phosphorylation, and citrullination, which may regulate its chromatin binding dynamics (Keywords).
• As a developmental and differentiation marker, H1t expression is tightly linked to spermatogenesis, serving as an indicator of meiotic and post-meiotic stages in testis.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 150-207 of human Histone H1t (C-terminal region). This design may enable detection of full-length H1t and possibly reveal C-terminal-specific modifications or isoforms. For unambiguous isoform identification, consider pairing with an N-terminal-specific antibody.
• For Western blot applications, using nuclear extracts from testis tissue or cell lines known to express H1t (e.g., spermatocytes) is recommended, along with appropriate loading controls and molecular weight markers to confirm the ~22 kDa band.
• In immunohistochemistry-paraffin (IHC-P), testis sections serve as ideal positive controls due to the tissue-specific expression. Antigen retrieval conditions may influence staining; optimize using standard citrate or EDTA buffers and validate with known positive/negative tissues.
• Given cross-reactivity with mouse and rat, this antibody can be utilized in rodent models; however, sequence alignment of the immunogen region across species is advisable to predict recognition patterns.
• ELISA performance should be verified by coating with the immunizing peptide or recombinant H1t protein and comparing signal-to-noise ratios against suitable blank controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibodies, including ready-to-use pairs and optimized monoclonals/polyclonals like this anti-Histone H1t antibody. We support diagnostic manufacturers and research teams with bulk ancillary reagents, custom sourcing for rare targets, and technical consultation—all designed to accelerate your assay development from concept to clinic. Whether you need chromatin biology markers or spermatogenesis-related reagents, CamelBio serves as your reliable, integrated supply partner.