Anti-Histone H1.4 Rabbit Polyclonal Antibody for WB and ELISA - P10412

Core Product Specifications and Parameters

Biological Background: Histone H1.4 Function and Localization

• Histone H1.4 (also known as Histone H1b or H1s-4) is a linker histone variant encoded by the H1-4 gene (formerly HIST1H1E) in humans.
• It binds to linker DNA between nucleosomes, facilitating the formation of higher-order chromatin structures and the 30 nm fiber. Related references: PMID:35581345 PMID:40240600
• H1.4 promotes H3K27me3 deposition by the PRC2/EED-EZH2 complex, contributing to gene silencing. Related references: PMID:35581345 PMID:40240600 PMID:40516528
• The protein's ability to compact chromatin is influenced by linker DNA length and trajectory. Related references: PMID:35581345
• Beyond structural roles, H1.4 regulates transcription through chromatin remodeling, nucleosome spacing, and DNA methylation. Related references: PMID:40240600
• Histone H1.4 is localized exclusively in the nucleus and on chromosomes.
• It undergoes numerous post-translational modifications including phosphorylation, acetylation, methylation, citrullination, and ADP-ribosylation, which modulate its function.
• The UniProt record documents experimentally observed modifications and disease associations such as intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human Histone H1.4. For applications, consider using a blocking peptide or alternative epitope verification to confirm specificity.
• For western blotting, expect a band at approximately 22 kDa in nuclear extracts from human, mouse, or rat samples. Optimal loading and gel conditions may require testing due to the highly basic nature of histone H1 proteins.
• In ELISA, direct or indirect coating with the immunogen peptide or recombinant Histone H1.4 can be used to assess antibody binding. Validate linearity and sensitivity in your experimental system.
• Since histone H1.4 is subject to various PTMs, experimental conditions (e.g., phosphatase inhibitors) may influence detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a complete portfolio of IVD raw materials, including validated antibody pairs, polyclonal antibodies, and ancillary reagents. For histone H1.4-related projects, we offer convenient access to high-quality products and technical guidance, helping you accelerate assay development and scale-up. From single components to full workflow solutions, CamelBio is your trusted partner from concept to clinic.