Anti-Histone H1.2 Monoclonal Antibody for WB, IF/ICC, ELISA - P16403

Core Product Specifications and Parameters

Biological Background: Histone H1.2 Function and Localization

• Core chromatin condensation: Histone H1.2 binds linker DNA between nucleosomes to form the chromatin fiber and is required for the condensation of nucleosome chains into higher-order structured fibers. Its absence leads to global chromatin decompaction (PubMed:26581166, 38530350). Related references: PMID:26581166 PMID:38530350
• Histone reader function: Specifically recognizes and binds histone H3 trimethylated at lysine 27 (H3K27me3) (PubMed:26581166). Related references: PMID:26581166
• PRC2-mediated H3K27me3 deposition: Promotes the formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex, likely facilitating restoration of H3K27me3 after replication (PubMed:37429872, 40516528). Related references: PMID:37429872 PMID:40516528
• Regulation of splicing: Acts as a regulator of splicing, particularly exon skipping and intron retention events, by binding to exons with high affinity and affecting RNA polymerase II elongation (PubMed:37922872). Related references: PMID:37922872
• Transcriptional regulation: Regulates individual gene transcription through chromatin remodeling, nucleosome spacing, and DNA methylation (PubMed:38530350). Related references: PMID:38530350
• Subcellular localization: Found in the nucleus, specifically in the nucleolus and on chromosomes.
• Post-translational modifications: Acetylation, ADP-ribosylation, citrullination, hydroxylation, methylation, and phosphorylation have been identified by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human Histone H1.2 (P16403), targeting the N-terminal region. Consider using the peptide antigen as a positive control in ELISA.
• For Western blot applications, denature samples under reducing conditions and optimize blocking and antibody dilution to account for the highly basic nature and small size (21 kDa) of histone H1.2.
• In immunofluorescence (IF/ICC), include a permeabilization step to allow nuclear access, and co-stain with a nuclear marker (e.g., DAPI) to confirm localization. Validate the antibody in cell lines or tissues known to express Histone H1.2.
• When using ELISA, consider that the sensitivity may depend on the native conformation of the protein; recombinant or peptide antigen may be suitable for standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive procurement channel for IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-Histone H1.2 monoclonal antibody supports investigations into chromatin biology, epigenetics, and gene regulation. We also specialize in sourcing rare-target raw materials to accelerate your assay development from concept to clinic.