Anti-Histone H1.2 (HIST1H1C) Rabbit Polyclonal Antibody - P16403

Core Product Specifications and Parameters

Biological Background: Histone H1.2 Function and Localization

• Protein identity: Histone H1.2 (also known as H1c, H1d, H1s-1) is a member of the histone H1 family, encoded by the H1-2 gene (HIST1H1C) in humans.
• Chromatin compaction: Histone H1.2 binds linker DNA to form the chromatin fiber and is essential for condensation of nucleosome chains into higher-order structures; its loss leads to global chromatin decompaction. Related references: PMID:26581166, PMID:38530350
• Histone reader activity: Specifically recognizes and binds histone H3 trimethylated at lysine 27 (H3K27me3). Related references: PMID:26581166
• Role in Polycomb repression: Promotes H3K27me3 methylation by the PRC2 complex, facilitating epigenetic maintenance. Related references: PMID:37429872, PMID:40516528
• Splicing regulation: Together with histone H1.3, regulates alternative splicing, particularly exon skipping and intron retention, by modulating RNA polymerase II elongation. Related references: PMID:37922872
• Gene transcription modulation: Regulates individual gene transcription through chromatin remodeling, nucleosome spacing, and DNA methylation. Related references: PMID:38530350
• Subcellular localization: Predominantly nuclear, enriched in the nucleolus and chromosomes.
• Post-translational modifications: Subject to acetylation, methylation, phosphorylation, citrullination, ADP-ribosylation, and hydroxylation, which modulate chromatin-binding affinity and function.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 100-200 of human HIST1H1C, a region within the globular domain. Verify specificity in your experimental system, as this domain is conserved among histone H1 variants.
• For Western blotting, expect a band at approximately 21 kDa. Optimize blocking and washing steps to minimize nonspecific binding.
• In IHC-P and IF/ICC, nuclear staining should be observed. Use appropriate antigen retrieval and fixation protocols.
• For ELISA, this antibody may be used for detection or capture; validate compatibility when developing a matched pair.
• Cross-reactivity with mouse and rat is reported; confirm species specificity with control lysates and recombinant proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting research institutes and diagnostic manufacturers with tailored solutions. Our polyclonal antibody against histone H1.2 aids chromatin and epigenetic studies, from basic research to assay development. We supply validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your project from concept to clinic.