Anti-Histone H1.0 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P07305

Core Product Specifications and Parameters

Biological Background: Histone H1.0 Function and Localization

• Histone H1.0, also known as H1' or H1(0), is a member of the linker histone H1 family that binds to linker DNA between nucleosomes, facilitating the formation of higher-order chromatin fibers and the macromolecular chromatin architecture. Related references: PMID:33238161
• H1.0 promotes the establishment of the repressive H3K27me3 histone mark by interacting with the PRC2/EED-EZH2 complex, thereby contributing to gene silencing and heterochromatin maintenance. Related references: PMID:33238161
• This histone variant is predominantly expressed in cells that are in terminal stages of differentiation or have a low rate of cell division, serving as a marker for non-proliferative, differentiated states. Related references: PMID:7374750
• H1.0 localizes to the nucleus, with enrichment in the nucleolus and along chromosomal regions, consistent with its role in chromatin compaction.
• The protein is subject to various post‑translational modifications—including acetylation, phosphorylation, citrullination, and ADP‑ribosylation—that regulate its binding dynamics and chromatin‑related functions.
• Alternative splicing and RNA editing of the H1-0 gene yield transcript variants, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen used to generate this monoclonal antibody is an N‑terminal synthetic peptide (amino acids 1–100 of human H1.0); therefore, the epitope likely resides within this region, and the antibody may detect full‑length H1.0 as well as potential N‑terminal fragments.
• For Western blotting (WB), a band near 21 kDa is expected; consider titrating the antibody and using appropriate loading controls for your cell or tissue lysates.
• In immunohistochemistry (IHC‑P), optimization of antigen retrieval and antibody dilution is recommended, especially when analyzing samples with variable H1.0 expression.
• The antibody has been shown to cross‑react with mouse and is predicted to recognize a broad range of species; validation in the desired model system is advised prior to extensive use.
• As a monoclonal rabbit antibody, it offers consistent lot‑to‑lot reproducibility, making it suitable for quantitative assays such as ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high‑quality, validated reagents. This anti‑Histone H1.0 monoclonal antibody is one example of our extensive portfolio targeting nuclear and chromatin‑associated proteins, supporting applications in epigenetics, oncology, and cell differentiation studies. Beyond single antibodies, we supply validated antibody pairs, bulk ancillary reagents, and offer customized sourcing for rare‑target raw materials, streamlining your development from concept to clinical readiness.