Anti-Histidine Ammonia-Lyase (HAL) Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P42357

Core Product Specifications and Parameters

Biological Background: Histidine Ammonia-Lyase Function and Localization

• Encoded by the HAL gene, Histidine ammonia-lyase is a lyase enzyme directly involved in histidine metabolism.
• The protein belongs to the ammonia-lyase family and catalyzes the non-oxidative deamination of L-histidine to urocanic acid, initiating histidine degradation.
• As a phosphoprotein, its activity may be regulated by phosphorylation events, affecting metabolic flux through the histidine pathway.
• Multiple transcript variants are produced through alternative splicing, potentially giving rise to isoforms with distinct functional properties.
• Disease‑associated variants in HAL have been documented, linking the enzyme to inherited disorders of histidine metabolism.
• HAL is part of the reference proteome and has been confidently identified in comprehensive proteomics screens.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200); therefore, the polyclonal antibody may detect multiple isoforms generated by alternative splicing. Confirm the observed band pattern in your specific sample system.
• In Western blotting, a dominant band is expected near 73 kDa (the specified molecular weight), which aligns with the theoretical mass of approximately 72.7 kDa. Use appropriate loading controls and consider running a positive control lysate.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions, as antigen accessibility can vary between tissue types.
• The antibody is predicted to cross-react with human, mouse, and rat based on sequence homology of the immunogen region. Validate species reactivity in your experimental model before large‑scale use.
• Store the antibody as recommended upon arrival (ice bag shipping suggests cold-chain maintenance) and avoid repeated freeze–thaw cycles to preserve binding activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies like this HAL rabbit polyclonal antibody. Backed by one-stop sourcing services that span from feasibility assessment to clinical application, we help streamline your supply chain for rare and routine targets alike. Whether you need customized antibody pairs, bulk ancillary reagents, or technical consulting, CamelBio is your bridge from concept to clinic.