Anti-Histamine H4 Receptor (HRH4) Polyclonal Antibody for WB and ELISA - Q9H3N8

Core Product Specifications and Parameters

Biological Background: Histamine H4 Receptor Function and Localization

• Histamine H4 receptor (HRH4) is a G protein-coupled receptor encoded by the HRH4 gene, with alternative splicing isoforms reported.
• HRH4 mediates chemotaxis of immune cells such as mast cells, eosinophils, basophils, dendritic cells, and neutrophils toward sites of inflammation by responding to histamine (PubMed:26948974). Related references: PMID:26948974
• Activation triggers release of proinflammatory cytokines including TNF, CCL4, IL6, and IFN-gamma, contributing to immune and inflammatory responses (PubMed:29600327, PubMed:31583075). Related references: PMID:29600327 PMID:31583075
• Ligand binding induces a conformational change that signals via G(i)/GNAI1, inhibiting adenylate cyclase and decreasing intracellular cAMP levels (PubMed:39333117). Related references: PMID:39333117
• The receptor is located primarily at the cell membrane, consistent with its role as a transmembrane signaling receptor.
• HRH4 is expressed predominantly in bone marrow and immune cells including T-cells, dendritic cells, monocytes, mast cells, neutrophils, and basophils, but also found in peripheral tissues such as heart, kidney, liver, lung, and spleen (PubMed:12503632, PubMed:26948974, PubMed:29600327). Related references: PMID:12503632 PMID:26948974 PMID:29600327
• Post-translational modifications include glycosylation and disulfide bond formation, typical of GPCRs, and the protein has a molecular weight of approximately 44 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 194–304 of human HRH4, likely covering the third intracellular loop and C-terminal tail. This may influence epitope accessibility in native versus denaturing conditions.
• For Western blot (WB) analysis, consider including appropriate controls such as HRH4 knockout or knockdown lysates to verify band specificity, given the reported molecular weight around 44 kDa.
• ELISA applications may benefit from using the recombinant protein immunogen as a positive control to optimize coating and detection conditions.
• Observed cross-reactivity with mouse and rat suggests that the epitope sequence is conserved across these species; however, validate detection in the relevant sample system to confirm performance.

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CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supplying validated antibody pairs, bulk ancillary reagents, and rare-target antibodies like this HRH4 polyclonal antibody to support immunology and inflammation research. With our one-stop access to optimized monoclonal and polyclonal antibodies, diagnostic manufacturers and labs can streamline supply chain challenges from concept to clinical application. We are dedicated to bridging your procurement needs for high-quality raw materials in immunoassay development and beyond.